A 3D-printed, multi-modal microfluidic device for measuring nitric oxide and ATP release from flowing red blood cells.
A 3D-printed, multi-modal microfluidic device for measuring nitric oxide and ATP release from flowing red blood cells.
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DOI：
10.1039/d2ay00931e
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发表时间：
2022-08-25
影响因子：
3.1
通讯作者：
Martin, R. Scott
中科院分区：
文献类型：
--
作者：
Hayter, Elizabeth A.;Azibere, Samuel;Skrajewski, Lauren A.;Soule, Logan D.;Spence, Dana M.;Martin, R. Scott
在本文中，设计并制造了一种3D打印多模式装置，用于同时检测全血制备的红细胞悬浮液中的一氧化氮（NO）和三磷酸腺苷（ATP）。将样品注入设备后，首先使用带有铂黑/Nafion 涂层金工作电极的三电极双对置电极配置检测 NO（通过电流分析法）。在线安培检测 NO 后，通过化学发光反应检测 ATP，将荧光素/荧光素酶溶液连续泵入集成混合 T，并使用通道下方的 PMT 测量产生的光。该设备针对混合/反应条件、检测限（NO 为 40 nM，ATP 为 30 nM）和灵敏度进行了优化。该装置用于测定红细胞中 NO 和 ATP 的基础（常氧）水平，以及缺氧条件下两种分析物浓度的增加。最后，还通过监测三周储存时间内 NO 和 ATP 的产生来研究将红细胞储存在常用储存溶液中的效果。开发了一种 3D 打印多模式设备，可同时检测红细胞悬浮液中的一氧化氮 (NO) 和三磷酸腺苷 (ATP)。
In this paper, a 3D-printed multi-modal device was designed and fabricated to simultaneously detect nitric oxide (NO) and adenosine triphosphate (ATP) in red blood cell suspensions prepared from whole blood. Once a sample was injected into the device, NO was first detected (via amperometry) using a three-electrode, dual-opposed, electrode configuration with a platinum-black/Nafion coated gold working electrode. After in-line amperometric detection of NO, ATP was detected via a chemiluminescence reaction, with a luciferin/luciferase solution continuously pumped into an integrated mixing T and the resulting light being measured with a PMT underneath the channel. The device was optimized for mixing/reaction conditions, limits of detection (40 nM for NO and 30 nM for ATP), and sensitivity. This device was used to determine the basal (normoxic) levels of NO and ATP in red blood cells, as well as an increase in concentration of both analytes under hypoxic conditions. Finally, the effect of storing red blood cells in a commonly used storage solution was also investigated by monitoring the production of NO and ATP over a three-week storage time. A 3D-printed multi-modal device was developed to simultaneously detect nitric oxide (NO) and adenosine triphosphate (ATP) in red blood cell suspensions.
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影响因子：
3.7
作者：
Eligini S;Porro B;Lualdi A;Squellerio I;Veglia F;Chiorino E;Crisci M;Garlaschè A;Giovannardi M;Werba JP;Tremoli E;Cavalca V
通讯作者：
Cavalca V
影响因子：
3.3
作者：
Giustarini, D;Dalle-Donne, I;Rossi, R
通讯作者：
Rossi, R
DOI：
10.1016/s0006-291x(05)81154-4
发表时间：
1991-10-31
影响因子：
3.1
作者：
BOGLE, RG;COADE, SB;MANN, GE
通讯作者：
MANN, GE
影响因子：
82.9
作者：
Cosby, K;Partovi, KS;Gladwin, MT
通讯作者：
Gladwin, MT
红细胞：氧传感器和血管张力调节器。
影响因子：
8.4
作者：
Ellsworth, Mary L.;Ellis, Christopher G.;Goldman, Daniel;Stephenson, Alan H.;Dietrich, Hans H.;Sprague, Randy S.
通讯作者：
Sprague, Randy S.