A novel one-step quick assay for detection of SARS-COV2 antibodies across mammalian species.
A novel one-step quick assay for detection of SARS-COV2 antibodies across mammalian species.
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作者：
Zhou X
到目前为止，严重急性呼吸综合征冠状病毒2(SARS-CoV2)已经感染了近1亿人，导致全球100多万人死亡。通过鉴定SARS-CoV2病毒的抗体，已经开发了许多血清学试验来追踪社区中的病毒感染情况。然而，这些测试在敏感性、特异性、复杂性和速度上各不相同。在这里，我开发了一种简单、一步、快速的检测方法，通过直接观察抗原-抗体反应来检测针对SARS-CoV2N(SCN)核衣壳蛋白的抗体。SARS-CoV2患者血清标本共40份，均购自RayBiotech。共有50份来自圣地亚哥血库的大流行前人类血清样本作为阴性对照。在对这90份血清样本进行一步快速检测后，我发现39份样本的抗SCN抗体呈阳性。所有39例阳性患者都来自40名SARS-CoV2患者，这表明一步法比最广泛使用的快速抗体检测方法-横向流动免疫分析(LFIA)更敏感。在大流行前的50份样本中，没有一份抗SCN抗体呈阳性，这表明一步法具有极好的特异性。一步法检测抗体只需约5分钟；1毫升的大肠杆菌培养物可产生足以进行数千次检测的试剂蛋白。由于一步检测不需要二次抗体，它可以用作不同哺乳动物物种中抗SCN抗体的通用检测，以追踪人类感染和SARS-CoV2病毒的动物宿主。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) has so far infected almost a hundred of millions of people and caused more than a million of death across the world. Many serological tests have been developed to track down virus infection in community via identification of antibodies against SARS-CoV2 virus. However, the tests vary in sensitivity, specificity, complexity, and speed. Here, I developed a simple, one-step, quick test to detect antibodies against SARS-CoV2 N (scN) nucleocapsid protein via direct visualization of antigen-antibody reaction. A total of 40 serum samples of SARS-CoV2 patients were purchased from RayBiotech. A total of 50 pre-pandemic human serum samples from San Diego Blood Bank were used as negative controls. After performing the one-step quick test of these 90 serum samples, I found that 39 samples are positive for anti-scN antibodies. All of the 39 positives are from the 40 SARS-CoV2 patients, suggesting that the one-step test is more sensitive than the lateral flow immunoassay (LFIA), the most widely used rapid antibody test. None of the 50 pre-pandemic samples is positive for anti-scN antibodies, indicating that the one-step test has an excellent specificity. The one-step test takes only ~5 min to detect the antibodies; and 1 ml of Escherichia coli culture can produce reagent proteins sufficient for thousands of the tests. Since the one-step test does not need a secondary antibody, it can be used as a universal test for anti-scN antibodies across different mammalian species to track down both human infection and the animal reservoir of SARS-CoV2 virus.
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影响因子：
3.9
作者：
Pavlova, Ina P.;Nair, Sujit S.;Tewari, Ash K.
通讯作者：
Tewari, Ash K.
影响因子：
12.7
作者：
Li, Zhengtu;Yi, Yongxiang;Ye, Feng
通讯作者：
Ye, Feng
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影响因子：
19.4
作者：
Dai, Bo;Jiao, Ziao;Zhang, Dawei
通讯作者：
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DOI：
10.1128/cdli.10.4.506-513.2003
发表时间：
2003-07-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Peruski, AH;Peruski, LF
通讯作者：
Peruski, LF