Chronic treatment with fluoride affects the jejunum: insights from proteomics and enteric innervation analysis.
Chronic treatment with fluoride affects the jejunum: insights from proteomics and enteric innervation analysis.
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DOI：
10.1038/s41598-018-21533-4
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发表时间：
2018-02-16
影响因子：
4.6
通讯作者：
Buzalaf MAR
中科院分区：
文献类型：
--
作者：
Dionizio AS;Melo CGS;Sabino-Arias IT;Ventura TMS;Leite AL;Souza SRG;Santos EX;Heubel AD;Souza JG;Perles JVCM;Zanoni JN;Buzalaf MAR
胃肠道症状是氟化物（F）中毒的第一个迹象。在本研究中，通过蛋白质组学和形态学分析对长期暴露于氟的大鼠空肠进行了评价。Wistar大鼠连续30天饮用含0、10和50 mgF/L的水。免疫荧光法检测空肠肌间神经丛中HuC/D、神经元型一氧化氮（nNOS）、血管活性肠肽（VIP）、降钙素基因相关肽（CGRP）和P物质（SP）的表达。与对照组和10 mgF/L组相比，高浓度氟处理组nNOS-IR神经元密度显著降低，VIP-IR神经元血管曲张程度显著增加。与对照组相比，F处理组的HUC/D-IR神经元密度和SP-IR静脉曲张面积均发生了显著的形态学变化。蛋白质组学揭示了与蛋白质合成、葡萄糖稳态和能量代谢等相关生物过程相关的各种蛋白质丰度的变化。
Gastrointestinal symptoms are the first signs of fluoride (F) toxicity. In the present study, the jejunum of rats chronically exposed to F was evaluated by proteomics, as well as by morphological analysis. Wistar rats received water containing 0, 10 or 50 mgF/L during 30 days. HuC/D, neuronal Nitric Oxide (nNOS), Vasoactive Intestinal Peptide (VIP), Calcitonin Gene Related Peptide (CGRP), and Substance P (SP) were detected in the myenteric plexus of the jejunum by immunofluorescence. The density of nNOS-IR neurons was significantly decreased (compared to both control and 10 mgF/L groups), while the VIP-IR varicosities were significantly increased (compared to control) in the group treated with the highest F concentration. Significant morphological changes were seen observed in the density of HUC/D-IR neurons and in the area of SP-IR varicosities for F-treated groups compared to control. Changes in the abundance of various proteins correlated with relevant biological processes, such as protein synthesis, glucose homeostasis and energy metabolism were revealed by proteomics.
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影响因子：
4.2
作者：
Bucci C;Alifano P;Cogli L
通讯作者：
Cogli L
影响因子：
3.7
作者：
Bottermann K;Reinartz M;Barsoum M;Kötter S;Gödecke A
通讯作者：
Gödecke A
影响因子：
24.5
作者：
Ekelund, KM;Ekblad, E
通讯作者：
Ekblad, E
影响因子：
4.1
作者：
Brewer, Paul Duffield;Habtemichael, Estifanos N.;Mastick, Cynthia Corley
通讯作者：
Mastick, Cynthia Corley
DOI：
10.1093/bioinformatics/btp101
发表时间：
2009-04-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Bindea G;Mlecnik B;Hackl H;Charoentong P;Tosolini M;Kirilovsky A;Fridman WH;Pagès F;Trajanoski Z;Galon J
通讯作者：
Galon J