Characterization of the expression of gastrin-releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans.
Characterization of the expression of gastrin-releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans.
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DOI：
10.1002/cne.25376
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发表时间：
2022-11
影响因子：
2.5
通讯作者：
Sakamoto, Hirotaka
中科院分区：
文献类型：
--
作者：
Takanami, Keiko;Oti, Takumi;Kobayashi, Yasuhisa;Hasegawa, Koki;Ito, Takashi;Tsutsui, Naoaki;Ueda, Yasumasa;Carstens, Earl;Sakamoto, Tatsuya;Sakamoto, Hirotaka
关键词：
胃泌素释放肽（GRP）及其受体（GRPR）已被确定为啮齿动物脊髓和三叉神经躯体感觉系统的瘙痒介质。在灵长类动物中，很少有关于GRP/GRPR在脊髓感觉系统中表达或功能的报道，并且在三叉神经系统中几乎一无所知。本研究的目的是表征GRP和GRPR在三叉神经和脊髓体感系统的日本猕猴（Macaca fuscata）。从猕猴背根神经节（DRG）中分离出编码GRP的cDNA，其氨基酸序列在哺乳动物尤其是灵长类动物中高度保守。免疫组化结果显示，GRP主要表达于成年猕猴三叉神经节和背根神经节。在三叉神经脊束核尾侧（Sp 5C）和脊髓的浅层中观察到密集染色的GRP免疫反应（IR）纤维。相反，在三叉神经感觉主核和三叉神经脊束核的口部和极间部中很少观察到GRP-ir纤维。cDNA克隆、原位杂交和Western blot结果显示，GRPR mRNA和GRPR蛋白在猕猴脊髓背角和Sp 5C中均有表达。我们的蛋白质配体印迹和配体衍生物染色GRPR显示，GRP直接结合在猕猴Sp 5C和脊髓背角在啮齿类动物中报道。最后，在人脊髓背角也检测到GRP-ir纤维。用GRP和GRPR标记的脊髓和三叉神经瘙痒神经回路似乎也在灵长类动物中起作用。
Gastrin-releasing peptide (GRP) and its receptor (GRPR) have been identified as itch mediators in the spinal and trigeminal somatosensory systems in rodents. In primates, there are few reports of GRP/GRPR expression or function in the spinal sensory system and virtually nothing is known in the trigeminal system. The aim of the present study was to characterize GRP and GRPR in the trigeminal and spinal somatosensory system of Japanese macaque monkeys (Macaca fuscata). cDNA encoding GRP was isolated from the macaque dorsal root ganglion (DRG) and exhibited an amino acid sequence that was highly conserved among mammals and especially in primates. Immunohistochemical analysis demonstrated that GRP was expressed mainly in the small-sized trigeminal ganglion and DRG in adult macaque monkeys. Densely stained GRP-immunoreactive (ir) fibers were observed in superficial layers of the spinal trigeminal nucleus caudalis (Sp5C) and the spinal cord. In contrast, GRP-ir fibers were rarely observed in the principal sensory trigeminal nucleus and oral and interpolar divisions of the spinal trigeminal nucleus. cDNA cloning, in situ hybridization, and Western blot revealed substantial expression of GRPR mRNA and GRPR protein in the macaque spinal dorsal horn and Sp5C. Our Western ligand blot and ligand derivative stain for GRPR revealed that GRP directly bound in the macaque Sp5C and spinal dorsal horn as reported in rodents. Finally, GRP-ir fibers were also detected in the human spinal dorsal horn. The spinal and trigeminal itch neural circuits labeled with GRP and GRPR appear to function also in primates.
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影响因子：
1.7
作者：
Fullmer, JM;Riedl, MS;Elde, R
通讯作者：
Elde, R
影响因子：
4.6
作者：
Lee, Heeseung;Ko, Mei-Chuan
通讯作者：
Ko, Mei-Chuan
影响因子：
3.3
作者：
Liu XY;Wan L;Huo FQ;Barry DM;Li H;Zhao ZQ;Chen ZF
通讯作者：
Chen ZF
DOI：
10.1152/ajpregu.1996.271.1.r180
发表时间：
1996-07-01
影响因子：
2.8
作者：
Ladenheim, EE;Taylor, JE;Moran, TH
通讯作者：
Moran, TH
影响因子：
2.5
作者：
OLSZEWSKI, J
通讯作者：
OLSZEWSKI, J