Phylogenetic Position and Geographic Differentiation of the Japanese Dormouse, Glirulus japonicus, Revealed by Variations among rDNA, mtDNA and the Sry Gene
Phylogenetic Position and Geographic Differentiation of the Japanese Dormouse, Glirulus japonicus, Revealed by Variations among rDNA, mtDNA and the Sry Gene
复制标题
rDNA、mtDNA 和 Sry 基因变异揭示日本睡鼠 Glirulus japonicus 的系统发育位置和地理分化
作者：
Hitoshi Suzuki;S. Minato;S. Sakurai;K. Tsuchiya;Igor M. Fokin
摘要日本睡鼠（Glirulus）是该属的唯一现存谱系，已被分类为单一种，分布于日本的三个主要岛屿，即本州、四国和九州。然而，从福井、和歌山和高知县（日本西南部）收集的个体产生了与从山梨县和长野县（日本中部）收集的个体明显不同的核核糖体DNA（rDNA）间隔区限制性片段谱。这两个群体之间的序列差异估计平均为2.8%，这相当于大约200万年前的假定差异。以线粒体DNA（mtDNA）为代表，直接测序法测定了细胞色素B基因402个碱基的序列，估计两组间的序列差异程度为6.5- 7%。这两个地理群体之间的差异也是实质性的约300个碱基片段的序列从Y-连锁，性别决定基因座，Sry。为了评估日本睡鼠和Myoxidae家族成员之间的系统发育关系，我们比较了日本睡鼠与森林睡鼠（Dryomys nitedula）和普通睡鼠（Muscardinus avellanarius）的线粒体12 S rRNA基因序列，这两个大陆属被认为与Glirulus属密切相关。结果表明，日本睡鼠的序列不同于两个大陆物种的任何序列，差异的程度有点类似于大鼠（褐家鼠）和仓鼠（仓鼠）之间的差异。
Abstract The Japanese dormouse, Glirulus japonicus, is the only extant lineage that represents this genus and it has been classified as a single species distributed on the three main islands of Japan, namely Honshu, Shikoku and Kyushu. However, individuals collected from Fukui, Wakayama and Kochi Prefectures (southwestern part of Japan) yielded distinctly different profiles of restriction fragments of the nuclear ribosomal DNA (rDNA) spacer from those collected from Yamanashi and Nagano Prefectures (central Japan). The estimated sequence divergence between the two groups was 2.8% on average, which corresponds to a putative divergence some two million years ago. Representing mitochondrial DNA (mtDNA) sequences, 402 bases of cytochrome b gene were determined by direct sequencing and the estimated extent of the sequence divergence between the two groups was 6.5–7%. Differences between the two geographic groups were also substantial in the sequences of about 300 base-fragments from the Y-linked, sex-determining locus, Sry. To assess the phylogenetic relationships between the Japanese dormouse and members of the family Myoxidae, we compared sequences of mitochondrial 12S rRNA gene of Japanese dormice with those of the forest dormouse (Dryomys nitedula) and the common dormouse (Muscardinus avellanarius), two continental genera thought to be closely related to the genus Glirulus. The results showed that the sequences from Japanese dormice were distinct from any sequences of the two continental species and the extent of the differences were somewhat similar to that between the rat (Rattus norvegicus) and the hamster (Mesocricetus auratus).
影响因子：
10.7
作者：
C. Nagamine;T. Shiroishi;N. Miyashita;K. Tsuchiya;H. Ikeda;N. Takao;X. L. Wu;M. Jin;F. Wang;A. Kryukov
通讯作者：
C. Nagamine;T. Shiroishi;N. Miyashita;K. Tsuchiya;H. Ikeda;N. Takao;X. L. Wu;M. Jin;F. Wang;A. Kryukov
影响因子：
10.7
作者：
B. Lundrigan;P. Tucker
通讯作者：
B. Lundrigan;P. Tucker