Inhibited carnitine synthesis impairs adaptation to high-fat diet in Nile tilapia (Oreochromis niloticus)
Inhibited carnitine synthesis impairs adaptation to high-fat diet in Nile tilapia (Oreochromis niloticus)
复制标题
肉碱合成受到抑制会损害尼罗罗非鱼（Oreochromis niloticus）对高脂肪饮食的适应
DOI：
10.1016/j.aqrep.2019.100249
复制
发表时间：
2020-03
影响因子：
3.7
通讯作者：
Du Zhen-Yu
中科院分区：
文献类型：
--
作者：
Li Ling-Yu;Li Jia-Min;Qiao Fang;Chen Li-Qiao;Zhang Mei-Ling;Du Zhen-Yu
高脂饲料(HFD)对养殖鱼类有一定的不良影响，因此鱼类对HFD的适应是一个重要的研究课题。本研究旨在探讨肉碱及其相关的线粒体脂肪酸β氧化(FAO)在尼罗罗非鱼适应高脂饲料中的作用。尼罗罗非鱼饲喂高脂饲料(13%)、高脂饲料( + + MD，肉碱合成抑制剂，每天1000 mg/kg体重)或正常脂肪饲料(NFD，7%)，饲养8周。投喂试验结束后，饲喂HFD的鱼体表现出较高的肝脏游离肉碱含量和FAO活性，而血清甘油三酯(TG)和全身脂肪水平相近。然而，与高脂饲料组相比，高脂饲料 + MD组显著降低了组织中肉碱含量和粮农组织效率，提高了血清游离脂肪酸和甘油三酯、全身脂肪和肝脏甘油三酯的含量。此外，喂食HFD的鱼上调了与粮农组织、脂肪运输和脂肪分解相关的基因的表达。然而，饲喂HFD + MD的鱼显示出与脂肪分解和脂肪运输相关的基因转录水平较低，而脂肪生成基因较高。这些结果表明，饲料中添加MD消除了摄食HFD的鱼类的适应性变化，表明肉碱及其相关的FAO活性在鱼类对HFD的适应中起着重要作用。这项研究表明，在HFD在水产养殖中的实际应用中，内源肉碱浓度和线粒体FAO活性应该是重要的检查点。
High-fat diet (HFD) caused some adverse effects in farmed fish, thus the adaptation to HFD in fish is an important research topic. This study was conducted to examine the roles of carnitine and its related mitochondrial fatty acid β-oxidation (FAO) in the adaptation to HFD in Nile tilapia. Nile tilapias were fed with HFD (13 %), HFD + mildronate (HFD + MD, inhibitor of carnitine synthesis, 1000 mg/kg body per day) or a normal fat diet (NFD, 7 %) for eight weeks. After the feeding trail, the fish fed with HFD showed higher hepatic free carnitine content and FAO activities, and similar levels of serum triglyceride (TG) and whole body fat. However, the HFD + MD-fed fish remarkably decreased carnitine content and FAO efficiency in tissues than those in the HFD-fed fish, and increased contents of serum free fatty acids (FFA) and TG, whole body fat and hepatic TG. Moreover, the HFD-fed fish upregulated the expressions of the genes associated to FAO, lipid transport and lipolysis. Nevertheless, the fish fed with HFD + MD showed lower transcriptional levels of the genes related to lipolysis and lipid transport, and higher lipogenesis genes. These results indicate that the adaptive changes in the fish fed with HFD were eliminated by dietary MD supplementation, and show that carnitine and its related FAO activity play important roles in the adaptation to HFD in fish. This study illustrates that in the practical usage of HFD in aquaculture, the endogenous carnitine concentration and mitochondrial FAO activities should be important checkpoints.
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肉碱合成受到抑制导致斑马鱼营养代谢的系统性改变
影响因子：
4
作者：
Li JM;Li LY;Qin X;Degrace P;Demizieux L;Limbu SM;Wang X;Zhang ML;Li DL;Du ZY
通讯作者：
Du ZY
DOI：
10.1097/maj.0b013e3182688026
发表时间：
2013-09
期刊：
The American Journal of the Medical Sciences
影响因子：
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作者：
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通讯作者：
Mengliu Yang;Shengbing Li;Hua Liu;Gangyi Yang;Lingou Li
DOI：
10.1111/j.1742-7843.2009.00461.x
发表时间：
2009-12-01
影响因子：
3.1
作者：
Liepinsh, Edgars;Kuka, Janis;Dambrova, Maija
通讯作者：
Dambrova, Maija
草食性草鱼（Ctenopharyngodon idella）高水平利用不同膳食脂质来源：与肝脏脂肪酸氧化相关的机制
影响因子：
3.5
作者：
Du, Z. Y.;Clouet, P.;Liu, Y. J.
通讯作者：
Liu, Y. J.
影响因子：
4.5
作者：
Betancor, M. B.;Sprague, M.;Sayanova, O.;Usher, S.;Campbell, P. J.;Napier, J. A.;Caballero, M. J.;Tocher, D. R.
通讯作者：
Tocher, D. R.