Evaluation of a high-EPA oil from transgenic Camelina sativa in feeds for Atlantic salmon (Salmo salar L.): Effects on tissue fatty acid composition, histology and gene expression.
Evaluation of a high-EPA oil from transgenic Camelina sativa in feeds for Atlantic salmon (Salmo salar L.): Effects on tissue fatty acid composition, histology and gene expression.
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DOI：
10.1016/j.aquaculture.2015.03.020
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发表时间：
2015-07-01
期刊：
影响因子：
4.5
通讯作者：
Tocher, D. R.
中科院分区：
文献类型：
--
作者：
Betancor, M. B.;Sprague, M.;Sayanova, O.;Usher, S.;Campbell, P. J.;Napier, J. A.;Caballero, M. J.;Tocher, D. R.
目前，水产饲料中食用鱼油（FO）的一种替代品是不含ω-3（n-3）长链多不饱和脂肪酸（LC-PUFA）的植物油（VO）。通过从头产生的n-3 LC-PUFA如二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的新来源是填补这些重要营养素的供应和需求之间的差距的潜在解决方案。对亚麻荠进行代谢工程改造以产生具有> 20% EPA的种子油（ECO），并测试其替代大西洋鲑鱼饲料中的FO的潜力。以FO、野生型亚麻荠油（WCO）或ECO作为唯一脂质来源的三种实验饲料中的一种饲喂鱼7周。包括ECO没有影响任何性能参数的研究和增强的表观消化率的个别n-6和n-3 PUFA相比，饮食WCO。与喂食WCO的鱼相比，喂食ECO的鱼的脑、肝和肠（幽门盲囊）中保持高水平的EPA，并且肝脏和肠中DPA和DHA的水平增加，这可能是由于基于基因上调的LC-PUFA生物合成增加。喂食ECO的鱼表现出与WCO相似的肝细胞内轻微的脂质蓄积，尽管与喂食FO的鱼没有显著差异。少数基因的调节可归因于ECO的特定效应（311个特征），其中代谢是受影响最大的类别。来自转基因亚麻荠（ECO）的EPA油可用作FO的替代物，然而它是含有FO（EPA）和VO（18：2n-6）脂肪酸特征的混合油，其导致类似的混合代谢和生理反应。评价了来自转基因亚麻荠属的高EPA油（ECO）作为鲑鱼饲料中鱼油（FO）的替代品。与FO相比，ECO替代FO不影响鱼的性能，并且提高了脂肪酸的表观消化率。ECO喂养的鱼在其组织中保持高水平的EPA，并且在幽门盲囊中没有显示出主要的转录组学变化。ECO被证明是鲑鱼饲料中FO的潜在合适替代品。
Currently, one alternative for dietary fish oil (FO) in aquafeeds is vegetable oils (VO) that are devoid of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFAs). Entirely new sources of n-3 LC-PUFA such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids through de novo production are a potential solution to fill the gap between supply and demand of these important nutrients. Camelina sativa was metabolically engineered to produce a seed oil (ECO) with > 20% EPA and its potential to substitute for FO in Atlantic salmon feeds was tested. Fish were fed with one of the three experimental diets containing FO, wild-type camelina oil (WCO) or ECO as the sole lipid sources for 7 weeks. Inclusion of ECO did not affect any of the performance parameters studied and enhanced apparent digestibility of individual n-6 and n-3 PUFA compared to dietary WCO. High levels of EPA were maintained in brain, liver and intestine (pyloric caeca), and levels of DPA and DHA were increased in liver and intestine of fish fed ECO compared to fish fed WCO likely due to increased LC-PUFA biosynthesis based on up-regulation of the genes. Fish fed ECO showed slight lipid accumulation within hepatocytes similar to that with WCO, although not significantly different to fish fed FO. The regulation of a small number of genes could be attributed to the specific effect of ECO (311 features) with metabolism being the most affected category. The EPA oil from transgenic Camelina (ECO) could be used as a substitute for FO, however it is a hybrid oil containing both FO (EPA) and VO (18:2n-6) fatty acid signatures that resulted in similarly mixed metabolic and physiological responses. A high-EPA oil (ECO) derived from transgenic Camelina was evaluated as a substitute for fish oil (FO) in salmon feeds Substitution of FO by ECO did not affect fish performance and enhanced apparent digestibility of fatty acids compared to FO ECO-fed fish maintained high levels of EPA in their tissues and showed no major transcriptomic changes in pyloric caeca. ECO was demonstrated to be a potentially suitable substitute for FO in salmon feeds.
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来自转基因Camelina sativa的营养增强油有效地取代了鱼油，作为鱼油的来源。
影响因子：
4.6
作者：
Betancor MB;Sprague M;Usher S;Sayanova O;Campbell PJ;Napier JA;Tocher DR
通讯作者：
Tocher DR
影响因子：
4.2
作者：
Bell, JG;McEvoy, J;Sargent, JR
通讯作者：
Sargent, JR
影响因子：
4.5
作者：
AUSTRENG, E
通讯作者：
AUSTRENG, E
影响因子：
3.5
作者：
Figueiredo-Silva, A;Rocha, E;Valente, LMP
通讯作者：
Valente, LMP
影响因子：
3.5
作者：
Brodtkorb, T.;Rosenlund, G.;Lie, O.
通讯作者：
Lie, O.