Structural basis for the photoconversion of a phytochrome to the activated Pfr form.
Structural basis for the photoconversion of a phytochrome to the activated Pfr form.
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光敏色素是一组含有胆色素的光感受器，它们通过在吸收红光的基态Pr和吸收远红光的光活化态Pfr之间进行光互变的能力来调节植物和微生物中的许多光响应。虽然已经确定了Pr等几种光敏色素的结构，但对Pfr的结构及其如何启动信号传导知之甚少。在这里，我们描述的三维溶液结构的胆色素结合结构域的Pfr使用蓝细菌光敏色素从聚球藻OSB '。与预测相反，光诱导的旋转的A，而不是D吡咯环是在光转换过程中的发色团的主要运动。随后的蛋白质内的重排，然后影响内和域间的光敏色素二聚体内的接触位点。从我们的模型中，我们提出，光敏色素的作用是通过传播可逆的光驱动的构象变化的胆色素改变相邻的输出域之间的接触，在大多数光敏色素直接差分磷酸转移。
Phytochromes are a collection of bilin-containing photoreceptors that regulate numerous photoresponses in plants and microorganisms through their ability to photointerconvert between a red light-absorbing, ground state Pr and a far-red light-absorbing, photoactivated state Pfr. While the structures of several phytochromes as Pr have been determined, little is known about the structure of Pfr and how it initiates signaling. Here, we describe the three-dimensional solution structure of the bilin-binding domain as Pfr using the cyanobacterial phytochrome from Synechococcus OSB’. Contrary to predictions, light-induced rotation of the A but not the D pyrrole ring is the primary motion of the chromophore during photoconversion. Subsequent rearrangements within the protein then affect intra- and interdomain contact sites within the phytochrome dimer. From our models, we propose that phytochromes act by propagating reversible light-driven conformational changes in the bilin to altered contacts between the adjacent output domains, which in most phytochromes direct differential phosphotransfer.
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影响因子：
4.1
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Karniol, B;Wagner, JR;Vierstra, RD
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RAPOPORT, H
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