Survival and hsp70 gene expression in Plutella xylostella and its larval parasitoid Diadegma insulare varied between slowly ramping and abrupt extreme temperature regimes.
Survival and hsp70 gene expression in Plutella xylostella and its larval parasitoid Diadegma insulare varied between slowly ramping and abrupt extreme temperature regimes.
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DOI：
10.1371/journal.pone.0073901
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Dosdall L
中科院分区：
文献类型：
--
作者：
Bahar MH;Hegedus D;Soroka J;Coutu C;Bekkaoui D;Dosdall L
在自然界中，昆虫已经进化出行为和生理上的适应，以应对短期暴露在极端温度下。由于气候变化，极端高温事件可能会增加；这反过来又会影响昆虫种群动态。研究了突然和生态相关的逐渐高温暴露对小菜蛾（diamondback moth， DBM）成虫和拟寄生物岛斑蝶（Diadegma insulare）以及被寄生和未被寄生的小菜蛾幼虫的存活和hsp70基因表达的影响。白杨成虫对高温的耐受性高于白杨成虫。insulare成年人。从25°C急剧升高到38°C， DBM成虫的存活率没有差异；然而，在40℃时，随着温度的逐渐升高，存活率更高。相反，更多的D。当温度升高到38°C和40°C时，岛状成虫存活下来。在高达40°C的高温胁迫下，寄生和未寄生的DBM幼虫的存活均无明显影响。在这两个物种的成虫中，当温度突然升高到38°C时，hsp70的表达比升温时更多。相比之下，在40°C时，暴露于斜坡而不是突变状态的昆虫中发现的表达量显著增加。Hsp70表达水平与成人生存数据一致，似乎是应激水平的良好指标。两种温度下，突变后的hsp70表达量均显著高于突变后的hsp70表达量。Hsp70基因表达对极端温度均有响应。这可能是这些昆虫在极端温度下生存能力的基础。突变后的存活和hsp70表达明显不同于突变后的存活和hsp70表达，这表明在将实验室结果外推到自然现场情况之前必须考虑实验方案。
In nature, insects have evolved behavioural and physiological adaptations to cope with short term exposure to extreme temperatures. Extreme heat events may increase as a result of climate change; this in turn will affect insect population dynamics. We examined the effect of abrupt and ecologically relevant gradual exposure to high temperatures on the survival and hsp70 gene expression in diamondback moth (DBM) adults and the parasitoid Diadegma insulare , as well as in parasitized and non-parasitized DBM larvae. Tolerance to high temperatures in DBM adults was higher than in D . insulare adults. There was no difference in the survival of DBM adults between abrupt and ramped increases from 25 to 38°C; however, at 40°C survival was higher when the temperature increased gradually. In contrast, more D . insulare adults survived when the temperature was ramped rather than shifted abruptly to both 38 and 40°C. There was no heat stress effect of up to 40°C on the survival of either parasitized or non-parasitized DBM larvae. In adults of both species, more hsp70 expression was observed when temperatures increased abruptly to 38°C compared to ramping. In contrast, at 40°C significantly more expression was found in insects exposed to the ramping rather than the abrupt regime. Hsp70 expression level was in agreement with adult survival data and appears to be a good indicator of stress levels. In parasitized and non-parasitized larvae, hsp70 expression was significantly higher after abrupt shifts compared to ramping at both temperatures. Hsp70 gene expression was responsive to extreme temperatures in both DBM and D . insulare , which may underlie the ability of these insects to survive in extreme temperatures. Survival and hsp70 expression upon abrupt changes are distinctly different from those after ramping indicating that experimental protocol must be considered before extrapolating laboratory results to natural field situations.
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影响因子：
3.7
作者：
Overgaard, Johannes;Kristensen, Torsten Nygaard;Sorensen, Jesper Givskov
通讯作者：
Sorensen, Jesper Givskov
影响因子：
3
作者：
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影响因子：
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DOI：
10.1016/j.cbpb.2005.05.044
发表时间：
2005-06-01
影响因子：
2.3
作者：
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通讯作者：
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影响因子：
1.7
作者：
Bahar, M. D. H.;Soroka, Juliana J.;Dosdall, Lloyd M.
通讯作者：
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