Refractive index measurement of the mouse crystalline lens using optical coherence tomography.
Refractive index measurement of the mouse crystalline lens using optical coherence tomography.
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DOI：
10.1016/j.exer.2014.05.015
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发表时间：
2014-08
影响因子：
3.4
通讯作者：
Pardue, Machelle T.
中科院分区：
文献类型：
--
作者：
Chakraborty, Ranjay;Lacy, Kip D.;Tan, Christopher C.;Park, Han Na;Pardue, Machelle T.
近年来，利用小鼠模型进行屈光发育和近视研究的兴趣越来越大。晶状体透镜是小鼠眼睛的关键光学组件，其占据大于50%的眼空间，并且厚度随着年龄的增长而显著增加。然而，小鼠晶状体透镜的折射率变化知之甚少。在这项研究中，我们研究了两种不同的菌株，野生型（WT）和尼克斯突变体（NOB）在正常的视觉发育过程中或形觉剥夺后的小鼠晶状体透镜的厚度和折射率的变化。使用谱域光学相干断层扫描（SD-OCT）对离体透镜进行折射率和透镜厚度测量。使用SD-OCT对5个标准球晶状体进行的折射率测量与其已知折射率（制造商提供）的比较表明，SD-OCT在离体计算小鼠透镜折射率方面具有良好的精密度（类内相关系数为0.998，Bland-Altman重复性系数为0.116）。在正常的视觉发育过程中，透镜厚度随着年龄的增长而显著增加，对于三个不同的小鼠群体，4岁（双眼平均厚度; WT：1.78 ± 0.03，nob：1.79 ± 0.08 mm），10（WT：2.02 ± 0.05，nob：2.01 ± 0.04 mm）和16周（WT：2.12 ± 0.06，nob：2.09 ± 0.06 mm，p<0.001）。在任何年龄，两种品系之间的透镜厚度均无显著差异（p=0.557）。对于具有正常视力的小鼠，nob小鼠中的分离晶状体的折射率显著大于WT小鼠（所有年龄的平均值; WT：1.42 ± 0.01，nob：1.44 ± 0.001，p<0.001）。在使用颅骨安装的护目镜装置对右眼进行4周形觉剥夺后，与两种品系的未处理对照组（右眼和左眼的平均值）相比，在护目镜动物的右眼和左眼中观察到晶状体透镜变薄（p=0.052）。在形觉剥夺小鼠中，nob小鼠中戴护目镜动物和未戴护目镜的未处理对照组之间的透镜折射率存在显著差异，但WT小鼠中无显著差异（p=0.009）。与未处理的对照组（1.45 ± 0.02，p<0.05）相比，戴眼罩的nob小鼠的双眼具有显著更大的透镜折射率（戴眼罩，1.49 ± 0.01;对侧，1.47 ± 0.03）。本文的结果表明，小鼠眼睛的晶体透镜折射率存在遗传差异，并且小鼠的透镜折射率随着形觉剥夺而显著增加。需要对小鼠眼睛进行精确光学测量的研究应用在解释SD-OCT数据时应考虑这些透镜折射率。
In recent years, there has been a growing interest for using mouse models in refractive development and myopia research. The crystalline lens is a critical optical component of the mouse eye that occupies greater than 50% of the ocular space, and significant increases in thickness with age. However, changes in refractive index of the mouse crystalline lens are less known. In this study, we examined the changes in thickness and refractive index of the mouse crystalline lens for two different strains, wild-type (WT) and a nyx mutant (nob) over the course of normal visual development or after form deprivation. Refractive index and lens thickness measurements were made on ex vivo lens using spectral domain optical coherence tomography (SD-OCT). Comparison of refractive index measurements on 5 standard ball lenses using the SD-OCT and their known refractive indices (manufacturer provided) indicated good precision (intra-class correlation coefficient, 0.998 and Bland-Altman coefficient of repeatability, 0.116) of the SD-OCT to calculate mouse lens refractive index ex vivo. During normal visual development, lens thickness increased significantly with age for three different cohorts of mice, aged 4 (average thickness from both eyes; WT: 1.78 ± 0.03, nob: 1.79 ± 0.08 mm), 10 (WT: 2.02 ± 0.05, nob: 2.01 ± 0.04 mm) and 16 weeks (WT: 2.12 ± 0.06, nob: 2.09 ± 0.06 mm, p<0.001). Lens thickness was not significantly different between the two strains at any age (p=0.557). For mice with normal vision, refractive index for isolated crystalline lenses in nob mice was significantly greater than WT mice (mean for all ages; WT: 1.42 ± 0.01, nob: 1.44 ± 0.001, p<0.001). After 4 weeks of form deprivation to the right eye using a skull-mounted goggling apparatus, a thinning of the crystalline lens was observed in both right and left eyes of goggled animals compared to their naïve controls (average from both the right and the left eye) for both strains (p=0.052). In form deprived mice, lens refractive index was significantly different between the goggled animals and non-goggled naïve controls in nob mice, but not in WT mice (p=0.009). Both eyes of goggled nob mice had significantly greater lens refractive index (goggled, 1.49 ± 0.01; opposite, 1.47 ± 0.03) compared to their naïve controls (1.45 ± 0.02, p<0.05). The results presented here suggest that there are genetic differences in the crystalline lens refractive index of the mouse eye, and that the lens refractive index in mice significantly increase with form deprivation. Research applications requiring precise optical measurements of the mouse eye should take these lens refractive indices into account when interpreting SD-OCT data.
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DOI：
10.2307/2987937
发表时间：
1983-01-01
期刊：
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES D-THE STATISTICIAN
影响因子：
--
作者：
ALTMAN, DG;BLAND, JM
通讯作者：
BLAND, JM
影响因子：
1.8
作者：
Dubbelman, M;Van der Heijde, GL
通讯作者：
Van der Heijde, GL
影响因子：
4.4
作者：
Gregg, RG;Mukhopadhyay, S;Peachey, NS
通讯作者：
Peachey, NS
影响因子：
4.2
作者：
Kuroda, T;Fujikado, T;Mihashi, T
通讯作者：
Mihashi, T
影响因子：
1.8
作者：
CAMPBELL, MCW;HUGHES, A
通讯作者：
HUGHES, A