Long non-coding RNA Igf2as controls hepatocellular carcinoma progression through the ERK/MAPK signaling pathway.
Long non-coding RNA Igf2as controls hepatocellular carcinoma progression through the ERK/MAPK signaling pathway.
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DOI：
10.3892/ol.2017.6492
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发表时间：
2017-09
期刊：
影响因子：
2.9
通讯作者：
Wang YK
中科院分区：
文献类型：
--
作者：
Bao H;Guo CG;Qiu PC;Zhang XL;Dong Q;Wang YK
长链非编码RNA（lncRNA）在包括癌症在内的许多人类疾病中起重要作用。lncRNA的异常表达与许多肿瘤类型有关;然而，lncRNA功能的潜在机制尚未阐明。本研究主要集中在胰岛素样生长因子2反义1（Igf 2as），一个lncRNA的报告，在肝细胞癌（HCC）的差异表达。采用逆转录-定量聚合酶链反应（RT-PCR）检测肝癌细胞及组织中Igf 2as的表达水平。流式细胞术用于测定Igf 2as抑制后细胞凋亡的水平，蛋白质印迹分析用于鉴定改变的蛋白质表达水平。结果表明，Igf 2as在HCC细胞和组织中上调，并且使用靶向小干扰RNA（si-Igf 2as）抑制Igf 2as显著降低细胞增殖并增加细胞凋亡。Western blot分析表明，转染si-Igf 2as的细胞中细胞外信号调节激酶/丝裂原活化蛋白激酶（ERK/MAPK）信号通路受到抑制。此外，Igf 2as的敲低显著降低了细胞迁移。这些结果表明，lncRNA Igf 2as可能主要通过调节ERK/MAPK信号通路来控制肝细胞的进展。
Long non-coding RNAs (lncRNAs) serve an important role in numerous human diseases, including cancer. Abnormal expression of lncRNAs has been associated with a number of tumor types; however, the underlying mechanisms through which lncRNA functions have yet to be elucidated. The present study primarily focuses on insulin-like growth factor 2 antisense 1 (Igf2as), a lncRNA reported to be differentially expressed in hepatocellular carcinoma (HCC). Reverse transcription-quantitative polymerase chain reaction analysis was used to determine the level of Igf2as in HCC cells and tissues. Flow cytometry was used to determine the level of cell apoptosis following Igf2as suppression and western blot analysis was used to identify altered protein expression levels. The results demonstrated that Igf2as was upregulated in HCC cells and tissues, and that the inhibition of Igf2as using a targeted small interfering RNA (si-Igf2as), significantly decreased cell proliferation and increased apoptosis. Western blot analysis identified that the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) signaling pathway was inhibited in cells transfected with si-Igf2as. In addition, cell migration was markedly reduced by the knockdown of Igf2as. These results suggest that lncRNA Igf2as may control hepatocellular progression primarily through the regulation of the ERK/MAPK signaling pathway.
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DOI：
10.1080/13102818.2014.981776
发表时间：
2015-01-02
期刊：
Biotechnology, biotechnological equipment
影响因子：
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作者：
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作者：
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