Androgens promote the acquisition of maturation competence in bovine oocytes.
Androgens promote the acquisition of maturation competence in bovine oocytes.
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DOI：
10.1262/jrd.2014-161
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Miyano T
中科院分区：
文献类型：
--
作者：
Makita M;Miyano T
最近在小鼠中的研究表明，雄激素对正常卵泡发育很重要。然而，关于雄激素在大型动物卵母细胞生长中的作用的报道很少。本研究旨在探讨雄激素在牛卵母细胞体外生长中的作用。将从0.4 - 0.7 mm早期窦状卵泡收集的卵母细胞-颗粒细胞复合体（OGC）与17β-雌二醇（E2）和非芳香化雄激素双氢睾酮（DHT）培养14天。我们还研究了雄激素受体（AR）抑制剂，羟基芴醇，拮抗雄激素对卵母细胞的影响的能力。在生长培养过程中，OGC结构在仅含DHT的培养基中塌陷，而在E2存在下，OGC结构得以维持。在同时添加雄激素和E2的培养液中，卵母细胞的平均直径从95 μm增加到120 μm左右，大于仅添加E2的培养液（115 μm）。此外，在成熟培养中，与雄激素（A4或DHT）和E2一起生长的卵母细胞显示出比仅与E2一起生长的卵母细胞（32%）更高的中期II卵母细胞百分比（分别为63%或69%）。此外，这些成熟率降低羟基芴醇以剂量依赖性的方式。免疫组化结果显示，AR在早期有腔卵泡的卵母细胞和颗粒细胞中均有表达，颗粒细胞的胞核呈强阳性表达。总之，虽然E2支持OGC结构，但在体外生长培养过程中，额外的雄激素通过AR促进卵母细胞生长和减数分裂能力的获得。
Recent studies in mice suggest that androgens are important for normal follicle development. However, there have been few reports concerning the action of androgens in the growth of oocytes from large animals. The purpose of this study was to determine the roles of androgens in bovine oocyte growth in vitro. Oocyte-granulosa cell complexes (OGCs) collected from 0.4−0.7 mm early antral follicles were cultured for 14 days with 17β-estradiol (E2) and a non-aromatizable androgen, dihydrotestosterone (DHT). We also examined the ability of an androgen receptor (AR) inhibitor, hydroxyflutamide, to antagonize the effect of androgens on the oocytes. During growth culture, the OGC structures collapsed in the medium with DHT alone, while in the presence of E2, the OGC structures were maintained. In the medium with both androgens and E2, the mean diameter of oocytes was increased from 95 μm to around 120 μm, larger than those grown with E2 alone (115 μm). Also in the maturation culture, oocytes grown with androgens (A4 or DHT) and E2 showed higher percentages of metaphase II oocytes (63% or 69%, respectively) than those grown with E2 alone (32%). Moreover, these maturation rates were decreased by hydroxyflutamide in a dose-dependent manner. Immunostaining showed that ARs were expressed in oocytes and granulosa cells in early antral follicles, and the nuclei of granulosa cells showed intense AR expression. In conclusion, although E2 supports the OGC structure, additional androgens promote oocyte growth and their acquisition of meiotic competence via AR during in vitro growth culture.
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影响因子：
--
作者：
Gill, A;Jamnongjit, M;Hammes, SR
通讯作者：
Hammes, SR
影响因子：
4.1
作者：
Berrevoets, CA;Umar, A;Brinkmann, AO
通讯作者：
Brinkmann, AO
影响因子：
3.6
作者：
BAGNELL, CA;MILLS, TM;MAHESH, VB
通讯作者：
MAHESH, VB
影响因子：
3.4
作者：
Adashi, EY
通讯作者：
Adashi, EY
DOI：
10.1073/pnas.0404372101
发表时间：
2004-08-03
影响因子：
11.1
作者：
Hu, YC;Wang, PH;Chang, CS
通讯作者：
Chang, CS