Resources to Facilitate Use of the Altered Schaedler Flora (ASF) Mouse Model to Study Microbiome Function.
Resources to Facilitate Use of the Altered Schaedler Flora (ASF) Mouse Model to Study Microbiome Function.
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DOI：
10.1128/msystems.00293-22
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发表时间：
2022-10-26
期刊：
影响因子：
6.4
通讯作者：
中科院分区：
文献类型：
--
作者：
定植有特定微生物群的动物代表了研究微生物组功能的有用实验系统。改变的谢德勒菌群 (ASF) 代表了八种鼠类细菌的联合体，这些细菌已经使用了 40 多年，需要对微生物群减少的小鼠进行研究。与无菌小鼠或仅定居一两个物种的小鼠相比，非洲猪瘟小鼠表现出正常的肠道结构和免疫系统发育。为了进一步扩大 ASF 的实用性，我们开发了技术和生物信息资源，以便能够使用该模型对微生物组功能进行基于系统的分析。在这里，我们重点介绍了这些资源的四种不同应用，这些应用能够实现并改进 (i) 通过定量 PCR 测量每个 ASF 成员的丰度； (ii) 对 ASF 基因组及其编码的构成整个肠道微生物组的代谢途径进行探索和比较分析； (iii) 全局转录谱，以确定其表达响应肠道内环境变化的基因； (iv) 发现因微生物群的进化适应而导致的遗传变化。这些资源旨在供广大研究人员使用，与传统饲养的小鼠（即具有复杂微生物组的小鼠）相结合，应有助于我们对微生物组结构和功能的理解。重要性需要改进的实验系统来加深我们对肠道微生物组如何影响哺乳动物宿主过程以及微生物群落结构和功能的理解。一种受到相当多关注的方法是使用具有已知组成的稳定微生物群（即确定的微生物群）的动物模型，这使得能够控制哺乳动物生物学的高度复杂和可变的特征。改变的谢德勒菌群 (ASF) 联盟是一个完善的明确微生物群模型，其中小鼠稳定地定居有 8 种不同的鼠科细菌物种。为了更好地利用 ASF，我们建立了新的实验和生物信息学资源，以便研究人员更好地利用该模型作为研究微生物组功能的实验系统。
Animals colonized with a defined microbiota represent useful experimental systems to investigate microbiome function. The altered Schaedler flora (ASF) represents a consortium of eight murine bacterial species that have been used for more than 4 decades where the study of mice with a reduced microbiota is desired. In contrast to germ-free mice, or mice colonized with only one or two species, ASF mice show the normal gut structure and immune system development. To further expand the utility of the ASF, we have developed technical and bioinformatic resources to enable a systems-based analysis of microbiome function using this model. Here, we highlighted four distinct applications of these resources that enable and improve (i) measurements of the abundance of each ASF member by quantitative PCR; (ii) exploration and comparative analysis of ASF genomes and the metabolic pathways they encode that comprise the entire gut microbiome; (iii) global transcriptional profiling to identify genes whose expression responds to environmental changes within the gut; and (iv) discovery of genetic changes resulting from the evolutionary adaptation of the microbiota. These resources were designed to be accessible to a broad community of researchers that, in combination with conventionally-reared mice (i.e., with complex microbiome), should contribute to our understanding of microbiome structure and function. IMPORTANCE Improved experimental systems are needed to advance our understanding of how the gut microbiome influences processes of the mammalian host as well as microbial community structure and function. An approach that is receiving considerable attention is the use of animal models that harbor a stable microbiota of known composition, i.e., defined microbiota, which enables control over an otherwise highly complex and variable feature of mammalian biology. The altered Schaedler flora (ASF) consortium is a well-established defined microbiota model, where mice are stably colonized with 8 distinct murine bacterial species. To take better advantage of the ASF, we established new experimental and bioinformatics resources for researchers to make better use of this model as an experimental system to study microbiome function.
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影响因子：
12.2
作者：
Becker, Natalie;Kunath, Julia;Blaut, Michael
通讯作者：
Blaut, Michael
DOI：
10.1084/jem.115.6.1161
发表时间：
1962-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
DUBOS RJ;SCHAEDLER RW
通讯作者：
SCHAEDLER RW
影响因子：
5
作者：
Dannemiller, Karen C.;Lang-Yona, Naama;Peccia, Jordan
通讯作者：
Peccia, Jordan
影响因子：
2.2
作者：
Biegert, Greyson;El Alam, Molly B.;Karpinets, Tatiana;Wu, Xiaogang;Sims, Travis T.;Yoshida-Court, Kyoko;Lynn, Erica J.;Yue, Jingyan;Medrano, Andrea Delgado;Petrosino, Joseph;Mezzari, Melissa P.;Ajami, Nadim J.;Solley, Travis;Ahmed-Kaddar, Mustapha;Klopp, Ann H.;Colbert, Lauren E.
通讯作者：
Colbert, Lauren E.
使用针对 groEL 基因的定量实时 PCR 对人类口腔和粪便副血链球菌进行定量
影响因子：
5.2
作者：
Chen, Qiurong;Wu, Guojun;Shen, Jian
通讯作者：
Shen, Jian