The Design and Characterization of a Strong Bio-Ink for Meniscus Regeneration.
The Design and Characterization of a Strong Bio-Ink for Meniscus Regeneration.
复制标题
用于半月板再生的强效生物墨水的设计与表征
DOI：
10.18063/ijb.v8i4.600
复制
发表时间：
2022
影响因子：
8.4
通讯作者：
Jiang, Qing
中科院分区：
文献类型：
--
作者：
Lu, Jingwei;Huang, Jianhao;Jin, Jing;Xie, Chunmei;Xue, Bin;Lai, Jiancheng;Cheng, Biao;Li, Lan;Jiang, Qing
半月板对于膝关节的机械功能至关重要，但由于承受重负荷而经常受到伤害。为未来的半月板组织工程制备了一种用于半月板再生的强生物墨水。制备的生物墨水由聚乙烯醇和脱细胞细胞外基质（PVA/dECM）组成。探讨了机械性能和流变特性，以评估冷冻/解冻循环和碱处理工艺的影响。使用三维打印机验证印刷适性。使用内皮细胞来评估生物相容性。最后建立12周兔半月板缺损模型来评价半月板再生能力。我们发现，在碱中浸泡 40 分钟和 20 次冷冻/解冻循环后的生物墨水表现出优异的机械性能。杨氏模量达到0.49 MPa，应力极限为2.9 MPa。结果还表明所提出的生物墨水在体外具有良好的可印刷性和生物相容性。 PVA/dECM 水凝胶在植入 12 周后治愈了半月板缺损。关节软骨和软骨下骨表现出正常的微观结构和成分。这些结果表明，PVA/dECM 水凝胶可能是修复半月板损伤的一种有前途的解决方案，对退行性半月板撕裂和创伤后关节炎具有预防作用。
The meniscus is vital to the mechanical function of the knee, while it is frequently harmed because it bears a heavy load. A strong bio-ink for meniscus regeneration was prepared for the future meniscal tissue engineering. The prepared bio-ink consists of poly (vinyl alcohol) and decellularized extracellular matrix (PVA/dECM). The mechanical properties and the rheological features were explored to evaluate the effects of freezing/thawing cycles and alkaline treatment process. The printability was verified using a three-dimensional printer. The endothelial cells were employed to assess the biocompatibility. Finally, a 12-week rabbit meniscus defect model was established to evaluate the meniscus regeneration capability. We found that the bio-ink by soaking in alkaline for 40 min and 20 freezing/thawing cycles demonstrated excellent mechanical properties. The Young’s modulus reached 0.49 MPa and the stress limitation was 2.9 MPa. The results also showed good printability and biocompatibility of the proposed bio-ink in vitro. The PVA/dECM hydrogel healed the meniscus defect after 12 weeks of implantation. The articular cartilage and subchondral bone exhibited normal microstructure and composition. These results suggested that the PVA/dECM hydrogel could be a promising solution to repair meniscal lesions with preventive effects against degenerative meniscal tears and post-traumatic arthritis.
登录
查看更多内容
影响因子：
5.4
作者：
Huang X;Guan N;Li Q
通讯作者：
Li Q
DOI：
10.1080/09205063.2018.1494437
发表时间：
2018-01-01
影响因子：
3.6
作者：
Parameswaran-Thankam, Anil;Al-Anbaky, Qudes;Ghosh, Anindya
通讯作者：
Ghosh, Anindya
DOI：
10.2106/00004623-198163010-00015
发表时间：
1981-01-01
影响因子：
5.3
作者：
LOTKE, PA;LEFKOE, RT;ECKER, ML
通讯作者：
ECKER, ML
影响因子：
9.6
作者：
NOBLE, J
通讯作者：
NOBLE, J
DOI：
10.1302/0301-620x.66b5.6548755
发表时间：
1984-01-01
影响因子：
--
作者：
ALLEN, PR;DENHAM, RA;SWAN, AV
通讯作者：
SWAN, AV