In vivo Evaluation of Mucociliary Clearance in Mice.
In vivo Evaluation of Mucociliary Clearance in Mice.
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DOI：
10.3791/61929
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发表时间：
2020-12-18
期刊：
影响因子：
--
通讯作者：
Zahid M
中科院分区：
文献类型：
--
作者：
Feldman KS;Zahid M
呼吸运动纤毛是细胞的特化细胞器，排列在呼吸道上皮细胞的顶面。通过以异时、同步的方式跳动，这些多个、能动的、基于肌动蛋白的细胞器产生头部流体流动，清除呼吸道中吸入的污染物和病原体。随着环境污染、新型病毒病原体和多重耐药细菌的出现，纤毛产生的粘膜纤毛清除（MCC）对于维持肺部健康至关重要。MCC也在多种先天性疾病如原发性纤毛运动障碍、囊性纤维化以及获得性疾病如慢性阻塞性肺病中被抑制。所有这些疾病都建立了小鼠模型，在某些情况下建立了多种小鼠模型。在这篇文章中，我们详细介绍了一种方法，使用少量的放射性和双模态SPECT/CT成像，以准确和可重复地测量MCC在小鼠体内。该方法允许在成像后恢复小鼠，使连续测量成为可能，并随时间纵向测试潜在的治疗剂。野生型小鼠的数据证明了MCC测量的重现性，只要足够注意细节，并严格遵守方案。
Respiratory motile cilia, specialized organelles of the cell, line the apical surface of epithelial cells lining the respiratory tract. By beating in a metachronal, synchronal fashion, these multiple, motile, actin-based organelles generate a cephalad fluid flow clearing the respiratory tract of inhaled pollutants and pathogens. With increasing environmental pollution, novel viral pathogens and emerging multi-drug resistant bacteria, cilia generated mucociliary clearance (MCC) is essential for maintaining lung health. MCC is also depressed in multiple congenital disorders like primary ciliary dyskinesia, cystic fibrosis as well as acquired disorders like chronic obstructive pulmonary disease. All these disorders have established, in some case multiple, mouse models. In this publication, we detail a method using a small amount of radioactivity and dual-modality SPECT/CT imaging to accurately and reproducibly measure MCC in mice in vivo. The method allows for recovery of mice after imaging, making serial measurements possible, and testing potential therapeutics longitudinally over time. The data in wild-type mice demonstrates the reproducibility of the MCC measurement as long as adequate attention to detail is paid, and the protocol strictly adhered to.
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影响因子：
3.3
作者：
Foster, WM;Walters, DM;Miller, LM
通讯作者：
Miller, LM
DOI：
10.1513/pats.200703-042br
发表时间：
2007-08-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Donaldson, Scott H;Corcoran, Timothy E;Bennett, William D
通讯作者：
Bennett, William D
影响因子：
37.8
作者：
Nakhleh N;Francis R;Giese RA;Tian X;Li Y;Zariwala MA;Yagi H;Khalifa O;Kureshi S;Chatterjee B;Sabol SL;Swisher M;Connelly PS;Daniels MP;Srinivasan A;Kuehl K;Kravitz N;Burns K;Sami I;Omran H;Barmada M;Olivier K;Chawla KK;Leigh M;Jonas R;Knowles M;Leatherbury L;Lo CW
通讯作者：
Lo CW
影响因子：
2.5
作者：
Donnelley, Martin;Morgan, Kaye S.;Parsons, David W.
通讯作者：
Parsons, David W.
DOI：
10.1083/jcb.201011152
发表时间：
2011-07-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carvalho-Santos Z;Azimzadeh J;Pereira-Leal JB;Bettencourt-Dias M
通讯作者：
Bettencourt-Dias M