AtPGK2, a member of PGKs gene family in Arabidopsis, has a positive role in salt stress tolerance
AtPGK2, a member of PGKs gene family in Arabidopsis, has a positive role in salt stress tolerance
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AtPGK2是拟南芥PGKs基因家族的成员，在耐盐胁迫中具有积极作用
DOI：
10.1007/s11240-014-0601-6
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Hou L
中科院分区：
文献类型：
--
作者：
Liu D;Li WC;Cheng JF;Hou L
磷酸甘油酸激酶（PGK）不仅在光合作用的碳代谢中起重要作用，而且在糖酵解和光合异生中也起重要作用。虽然已经从多种植物中克隆和纯化了大量PGKs，但其在非生物胁迫下的生理功能仍然不清楚。本研究从拟南芥中鉴定并鉴定了PGKs基因家族的一个成员AtPGK 2。序列分析表明，AtPGK 2与其他物种的PGK蛋白具有较高的序列相似性。通过定量逆转录聚合酶链反应（RT-PCR）和组织化学β-葡萄糖醛酸酶（β-glucuronidase，β-glucuronidase）分析，证明AtPGK 2主要在萌发的种子和花中表达，且其表达受盐胁迫的诱导。通过形态学和生理学分析，我们发现AtPGK 2的过量表达赋予了转基因拟南芥耐盐性。此外，AtPGK 2过表达植株表现出胁迫响应标记基因RD 29 A、RD 29 B、KIN 1和KIN 2的表达增强。以上结果表明，AtPGK 2基因在拟南芥中的过表达降低了植物对盐胁迫的敏感性，AtPGK 2基因可能在植物的耐盐性中起着积极的作用。据我们所知，这是首次对PGK在高等植物盐胁迫响应中的生理功能进行研究。
Phosphoglycerate kinase (PGK) plays a critical role not only in photosynthetic carbon metabolism but also in glycolysis and gluconeogenesis. Although a large number of PGKs have been cloned and purified from a variety of plant sources, their physiological functions in response to abiotic stresses still remain elusive. Here we identified and characterized a member of PGKs gene family,AtPGK2, fromArabidopsis thaliana. Sequence analysis showed that AtPGK2 had high sequence similarity to PGKs proteins from other species. By using quantitative reverse transcription-polymerase chain reaction and histochemical β-glucuronidase assays, we demonstrated thatAtPGK2was mainly expressed in germinating seeds and flowers, and it could be induced significantly by salt stress. Through morphological and physiological analyses, we found that over-expression ofAtPGK2conferred salt tolerance in transgenic Arabidopsis plants. Furthermore,AtPGK2-overexpressing plants showed enhanced expression of stress-responsive marker genes, includingRD29A,RD29B,KIN1andKIN2. Collectively, our results suggested that over-expression ofAtPGK2in Arabidopsis decreased plant sensitivity to salt stress, and AtPGK2 may play a positive role in salt stress tolerance. To our knowledge, this is the first study on the physiological functions of PGK in response to salt stress in higher plants.
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DOI：
10.1007/s11240-012-0219-5
发表时间：
2013-02
期刊：
Plant Cell, Tissue and Organ Culture
影响因子：
--
作者：
Yang, Chunwen;Deng, Wei;Tang, Ning;Wang, Xiaomin;Yan, Fang;Lin, Dongbo;Li, Zhengguo
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DOI：
10.3109/10425179809008459
发表时间：
1998
期刊：
DNA sequence : the journal of DNA sequencing and mapping
影响因子：
--
作者：
M. Löbler
通讯作者：
M. Löbler
DOI：
10.1104/pp.103.4.1449
发表时间：
1993-12
期刊：
--
影响因子：
--
作者：
U. Bertsch;T. Schlicher;I. Schroder;J. Soll
通讯作者：
U. Bertsch;T. Schlicher;I. Schroder;J. Soll
影响因子：
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作者：
KOPKESECUNDO, E;MOLNAR, I;SCHNARRENBERGER, C
通讯作者：
SCHNARRENBERGER, C
影响因子：
7.3
作者：
Crone, D;Rueda, J;Mascarenhas, JP
通讯作者：
Mascarenhas, JP