Effects of stimulation technique, anatomical region, and time on human sweat lipid mediator profiles.
Effects of stimulation technique, anatomical region, and time on human sweat lipid mediator profiles.
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DOI：
10.1016/j.prostaglandins.2017.09.007
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发表时间：
2018-01
影响因子：
2.9
通讯作者：
Newman JW
中科院分区：
文献类型：
--
作者：
Agrawal K;Waller JD;Pedersen TL;Newman JW
很少有研究比较采样协议对汗液成分的影响。在这里，我们评估汗液刺激模式和收集部位对脂质介质组成的影响。健康男性（n = 7）的汗液在毛果芸香碱离子电渗疗法或运动后每周从掌侧前臂收集，并且仅在毛果芸香碱离子电渗疗法后从前臂、背部和大腿收集，持续三周。通过液相色谱-串联质谱法测定汗液中150多种脂质介质的含量。常规检测70种脂质介质，包括前列腺素类、醇类、二醇类、环氧化物、酮类、硝基脂质、N-酰基乙醇酰胺、单酰基甘油和神经酰胺。检测到的脂质介质出现采样站点的影响，虽然前臂是最一致的来源的汗水。毛果芸香碱诱导的汗液中大多数检测到的化合物的浓度增加。此外，脂质介质浓度和分布在研究期间暂时稳定。因此，汗液似乎是一个一致的和解剖学上稳定的脂质介质的来源，但必须小心比较从不同的刺激技术获得的结果。
Few studies compare sampling protocol effect on sweat composition. Here we evaluate the impact of sweat stimulation mode and site of collection on lipid mediator composition. Sweat from healthy males (n = 7) was collected weekly for three weeks from the volar forearm following either pilocarpine iontophoresis or exercise, and from the forearm, back and thigh following pilocarpine iontophoresis only. Sweat content of over 150 lipid mediators were measured by liquid chromatography-tandem mass spectrometry. Seventy lipid mediators were routinely detected, including prostanoids, alcohols, diols, epoxides, ketones, nitrolipids, N-acylethanolamides, monoacylglycerols, and ceramides. Detected lipid mediators appeared unaffected by sampling site, though the forearm was the most consistent source of sweat. Pilocarpine-induced sweat showed increased concentrations of most detected compounds. Moreover, lipid mediator concentrations and profiles were temporally stable over the study duration. Sweat therefore appears to be a consistent and anatomically-stable source of lipid mediators, but care must be taken in comparing results obtained from different stimulation techniques.
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影响因子：
6.5
作者：
Iwata, C;Akimoto, N;Ito, A
通讯作者：
Ito, A
影响因子：
3.6
作者：
Harker, Mark;Coulson, Helen;Daykin, Clare A.
通讯作者：
Daykin, Clare A.
影响因子：
6.5
作者：
Agrawal, Karan;Hassoun, Lauren A.;Newman, John W.
通讯作者：
Newman, John W.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.7
作者：
Jergovic, Mladen;Bendelja, Kreso;Vidovic, Andelko
通讯作者：
Vidovic, Andelko