The acetylation level of rDNA in Brassica campestris

The acetylation level of rDNA in Brassica campestris
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油菜rDNA乙酰化水平

DOI:
10.1007/s12374-011-0317-7
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发表时间:
2012-06
影响因子:
2.9
通讯作者:
Lijia Li
Lijia Li
中科院分区:
生物学4区
文献类型:
--
作者:
Lu Zhang;Yong Hu;Shibin He;Lijia Li

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核糖体DNA是在间期形成核仁的重要重复序列。其转录成核糖体RNA用于核糖体生物合成也代表了几个细胞过程的过渡点,包括细胞周期进展、基因沉默和核糖核蛋白复合物的形成。rDNA乙酰化水平在调节rDNA染色质结构变化和转录活性中具有重要作用。利用油菜根尖样品,我们确定了一些rDNA染色质位于异染色质区域,而一些是去凝聚的,并发现在常染色质区域。免疫组化染色结果显示,组蛋白H4K5乙酰化和H4四乙酰化信号分散在常染色质中。通过染色质免疫沉淀(ChIP)分析rDNA的启动子和外显子区域揭示了组蛋白乙酰化与rDNA构象之间的联系。
Ribosomal DNA is an important repeated sequence that forms the nucleolus at the interphase. Its transcription into ribosomal RNA for ribosome biogenesis also represents a transitional point for several cellular processes, including cell-cycle progression, gene-silencing, and formation of the ribonucleoprotein complex. The levels of rDNA acetylation have an important role in regulating structural changes in rDNA chromatin and transcriptional activity. Using root-tip samples from Brassica campestris, we determined that some rDNA chromatin is located in the heterochromatin regions while some is de-condensed and found in euchromatin regions. Immuno-staining results showed that histone H4K5 acetylation and H4 tetra-acetylation signals are dispersed within the euchromatin. Analysis of the promoter and exon regions of rDNA via chromatin immuno-precipitation (ChIP) revealed a connection between histone acetylation and rDNA conformation.
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