The acetylation level of rDNA in Brassica campestris
The acetylation level of rDNA in Brassica campestris
复制标题
油菜rDNA乙酰化水平
DOI:
10.1007/s12374-011-0317-7
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发表时间:
2012-06
影响因子:
2.9
通讯作者:
Lijia Li
中科院分区:
文献类型:
--
作者:
Lu Zhang;Yong Hu;Shibin He;Lijia Li
Ribosomal DNA is an important repeated sequence that forms the nucleolus at the interphase. Its transcription into ribosomal RNA for ribosome biogenesis also represents a transitional point for several cellular processes, including cell-cycle progression, gene-silencing, and formation of the ribonucleoprotein complex. The levels of rDNA acetylation have an important role in regulating structural changes in rDNA chromatin and transcriptional activity. Using root-tip samples from Brassica campestris, we determined that some rDNA chromatin is located in the heterochromatin regions while some is de-condensed and found in euchromatin regions. Immuno-staining results showed that histone H4K5 acetylation and H4 tetra-acetylation signals are dispersed within the euchromatin. Analysis of the promoter and exon regions of rDNA via chromatin immuno-precipitation (ChIP) revealed a connection between histone acetylation and rDNA conformation.
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N. Pertsev
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