A new subfamily of short bacterial adenylate kinases with the Mycobacterium tuberculosis enzyme as a model: A predictive and experimental study
A new subfamily of short bacterial adenylate kinases with the Mycobacterium tuberculosis enzyme as a model: A predictive and experimental study
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以结核分枝杆菌酶为模型的短细菌腺苷酸激酶的新亚家族：预测和实验研究
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
C. Schultz
中科院分区：
文献类型：
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作者：
H. Munier;S. Burlacu;C. Craescu;H. Mantsch;C. Schultz
来自结核分枝杆菌的adk基因编码181个氨基酸的酶。与52种不同形式的腺苷酸激酶（AK）的序列比较表明，来自M.结核病属于一个新的“短”细菌AK亚家族。在大肠杆菌中过表达的重组蛋白表现出低催化活性和出乎意料的高热稳定性（Tm = 64.8°C）。基于各种光谱数据，对来自E. coli中，并对各种测序的AK的二级结构预测，我们提出了一个结构模型的AK从M。结核病（AKmt）。蛋白质1999;36：238-248.© 1999 Wiley‐利斯公司
The adk gene from Mycobacterium tuberculosis codes for an enzyme of 181 amino acids. A sequence comparison with 52 different forms of adenylate kinases (AK) suggests that the enzyme from M. tuberculosis belongs to a new subfamily of “short” bacterial AKs. The recombinant protein, overexpressed in Escherichia coli, exhibits a low catalytic activity and an unexpectedly high thermal stability (Tm = 64.8°C). Based on various spectroscopic data, on the known three‐dimensional structure of the AK from E. coli and on secondary structure predictions for various sequenced AKs, we propose a structural model for AK from M. tuberculosis (AKmt). Proteins 1999;36:238–248. © 1999 Wiley‐Liss, Inc.
影响因子：
14.9
作者：
RALEIGH, EA;MURRAY, NE;HANAHAN, D
通讯作者：
HANAHAN, D
影响因子：
56.9
作者：
Valdivia, RH;Falkow, S
通讯作者：
Falkow, S