Hypoxic contractile response in isolated rat thoracic aorta: role of endothelium, extracellular calcium and endothelin
Hypoxic contractile response in isolated rat thoracic aorta: role of endothelium, extracellular calcium and endothelin
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离体大鼠胸主动脉缺氧收缩反应：内皮、细胞外钙和内皮素的作用
DOI：
10.1111/j.1472-8206.1997.tb00178.x
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发表时间：
1997
影响因子：
2.9
通讯作者：
É. Bellissant
中科院分区：
文献类型：
--
作者：
D. Pape;J. Beuchard;P. Guillo;H. Allain;É. Bellissant
总结-缺氧对离体动脉的影响仍然存在争议，取决于物种，血管床和协议。本研究的目的是描述大鼠胸主动脉对缺氧的反应，并研究内皮细胞、细胞外钙离子和内皮素在这一反应中的作用。通过用95%N2和5%CO2代替95%O2和5%CO2鼓泡Krebs溶液来诱导缺氧。实验在去甲肾上腺素（0.01 μM）预收缩环中进行1小时。缺氧产生了一个双相反应，包括最初的短暂部分放松（67%，14分钟），然后缓慢但持续的收缩（27%，40至60分钟）。内皮细胞去除后，舒张出现更快，幅度增加（12分钟时为82%），随后是微弱的短暂收缩（25分钟时为16%）。在内皮完整的环中，无钙培养基（EGTA，0.1 mM）和钙通道阻滞剂维拉帕米（0.05、0.5和5 μM）或尼卡地平（0.1、1和10 μM）对舒张无影响，但抑制收缩，两种钙拮抗剂的作用均呈浓度依赖性。同样，ETA/ETB受体拮抗剂波生坦（0.1、10和1，000 nM）诱导收缩呈浓度依赖性降低。结论：1）大鼠胸主动脉在缺氧1 h时的反应是双相的（先舒张后收缩）; 2）内皮细胞参与收缩，而内皮细胞在舒张中的作用尚不清楚; 3）细胞外钙离子参与收缩; 4）内皮素可能在收缩中起作用。
Summary— The effects of hypoxia on isolated arteries remain controversial, depending on the species, vascular beds and protocols. The aims of the study were to characterize the response of rat thoracic aorta to hypoxia and to examine the roles of endothelium, extracellular calcium and endothelin in this response. Hypoxia was induced by bubbling Krebs solution with 95% N2 and 5% CO2 instead of 95% O2 and 5% CO2. Experiments were performed during 1 h in norepinephrine (0.01 μM) precontracted rings. Hypoxia produced a biphasic response consisting of an initial transient partial relaxation (67% at 14 min) followed by a slow but sustained contraction (27% from 40 to 60 min). After endothelium removal, relaxation appeared faster with increased magnitude (82% at 12 min) and was followed by a weak transient contraction (16% at 25 min). In endothelium‐intact rings, Ca2+ free medium (EGTA, 0.1 mM) and Ca2+ channel blockers, verapamil (0.05, 0.5 and 5 μM) or nicardipine (0.1, 1 and 10 μM), had no effect on relaxation but inhibited the contraction, the effects of both calcium antagonists being concentration‐dependent. Similarly, the ETA/ETB receptor antagonist, bosentan (0.1, 10 and 1,000 nM), induced a concentration‐dependent decrease in the contraction. We conclude that 1) the response of rat thoracic aorta during 1 h of hypoxia is biphasic (relaxation followed by contraction); 2) the endothelium is involved in the contraction whereas its role in the relaxation remains to be elucidated; 3) extracellular calcium is involved in the contraction; and 4) endothelin may play a role in the contraction.
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影响因子：
5.5
作者：
RUBANYI, GM;VANHOUTTE, PM
通讯作者：
VANHOUTTE, PM
影响因子：
3.3
作者：
Chen, SJ;Chen, YF;Oparil, S
通讯作者：
Oparil, S
影响因子：
--
作者：
GRASER, T;VANHOUTTE, PM
通讯作者：
VANHOUTTE, PM
缺氧对大鼠肺动脉和肠系膜动脉张力的对比影响。
DOI：
10.1152/ajpheart.1990.259.2.h281
发表时间：
1990
期刊：
The American journal of physiology
影响因子：
--
作者：
Yuan,XJ;Tod,ML;Rubin,LJ;Blaustein,MP
通讯作者：
Blaustein,MP
O2 和大鼠肺动脉张力：内皮、Ca2、氰化物和野百合碱的影响。
DOI：
10.1152/jappl.1991.71.1.30
发表时间：
1991
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Mathew,R;Burke-Wolin,T;Gewitz,MH;Wolin,MS
通讯作者：
Wolin,MS