A retrospective overview of enterovirus infection diagnosis and molecular epidemiology in the public hospitals of Marseille, France (1985-2005).
A retrospective overview of enterovirus infection diagnosis and molecular epidemiology in the public hospitals of Marseille, France (1985-2005).
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DOI：
10.1371/journal.pone.0018022
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发表时间：
2011-03-18
期刊：
影响因子：
3.7
通讯作者：
de Lamballerie X
中科院分区：
文献类型：
--
作者：
Tan CY;Ninove L;Gaudart J;Nougairede A;Zandotti C;Thirion-Perrier L;Charrel RN;de Lamballerie X
人类肠道病毒（HEV）是人类常见的病原体，特别是与无菌性脑膜炎的大规模爆发有关。在这里，我们编制了一个数据库的临床戊型肝炎病毒分离株从公立医院的马赛，从1985年至2005年。在654株可通过VP 1基因完全测序表征的分离株中，98%属于HEV-B种;最常见的分离血清型为埃可病毒E30、E11、E7、E6和E4。E30的高发病率和最近出现的E13与世界各地的报告一致，HEV分离高峰主要发生在春末和夏季。埃可病毒的比例逐年下降，而柯萨奇病毒的比例则有所增加。粪便（最常见的样本类型）可检出所有已鉴定的血清型。MRC 5（人肺成纤维细胞）细胞系是最有利于HEV分离的细胞系（10种最常见血清型分离株的84.9%，与BGM（布法罗绿色猴肾细胞）相关的96.3%）。与分子VP 1分析相比，先前基于血清中和的血清型鉴定证明了55.4%的准确性。我们对20多年来大量临床菌株的分析加强了VP 1血清分型的有效性，并表明比较p-距离得分可以与系统发育分析相结合，以提供明确的血清型鉴定。VP 1、2C和3D区域的系统发育分析也为临床分离株之间的重组事件提供了证据。特别是，它确定了不同的VP 1，但几乎相同的非结构区域的分离株。
Human enteroviruses (HEV) are frequent human pathogens and, associated in particular with large outbreaks of aseptic meningitis. Here, we have compiled a database of clinical HEV isolates from the Public Hospitals of Marseille, from 1985 to 2005. Amongst 654 isolates that could be characterized by complete sequencing of the VP1 gene, 98% belonged to species HEV-B; the most frequently isolated serotypes were Echovirus E30, E11, E7, E6 and E4. The high incidence of E30 and the recent emergence of E13 are consistent with reports worldwide and peak HEV isolation occurred mostly in the late spring and summer months. The proportion of echoviruses has decreased across the years, while that of coxsackieviruses has increased. Stool (the most frequent sample type) allowed detection of all identified serotypes. MRC5 (Human lung fibroblasts) cell line was the most conducive cell line for HEV isolation (84.9% of 10 most common serotype isolates, 96.3% in association with BGM (Buffalo green monkey kidney cells)). Previous seroneutralization-based serotype identification demonstrated 55.4% accuracy when compared with molecular VP1 analysis. Our analysis of a large number of clinical strains over 20 years reinforced the validity of VP1 serotyping and showed that comparative p-distance scores can be coupled with phylogenetic analysis to provide non-ambiguous serotype identification. Phylogenetic analysis in the VP1, 2C and 3D regions also provided evidence for recombination events amongst clinical isolates. In particular, it identified isolates with dissimilar VP1 but almost identical nonstructural regions.
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影响因子：
3.8
作者：
Lukashev, AN;Lashkevich, VA;Ilonen, J
通讯作者：
Ilonen, J
影响因子：
28.2
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Mizuta, K;Abiko, C;Murayama, S
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Murayama, S
影响因子：
8.8
作者：
Leitch, E. C. McWilliam;Harvala, H.;Simmonds, R.
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Simmonds, R.
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
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5.2
作者：
Chen, Tzu-Chun;Weng, Kuo-Feng;Shih, Shin-Ru
通讯作者：
Shih, Shin-Ru