Magnetic nanoparticle mediated enhancement of localized surface plasmon resonance for ultrasensitive bioanalytical assay in human blood plasma.
Magnetic nanoparticle mediated enhancement of localized surface plasmon resonance for ultrasensitive bioanalytical assay in human blood plasma.
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DOI：
10.1021/ac302422k
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发表时间：
2013-02-05
影响因子：
7.4
通讯作者：
Venkataramasubramani, Meenakshi
中科院分区：
文献类型：
--
作者：
Tang, Liang;Casas, Justin;Venkataramasubramani, Meenakshi
我们证明 Fe3O4 磁性纳米颗粒（MNP）可以极大地增强金属纳米颗粒的局域表面等离子体共振（LSPR）。 Fe3O4 MNP 的高折射率和分子量使其成为生物结合事件等离激元响应的强大增强剂，从而显着提高微量小分子 LSPR 测定的灵敏度、可靠性、动态范围和校准线性。这项研究没有使用荧光光谱或磁共振成像，而是首次使用无标记的 LSPR 纳米传感器来检测生理溶液中的疾病生物标志物，从而提供低成本、面向临床的检测。这种简便、超灵敏的纳米传感器具有极轻、坚固且低成本的仪器，对于芯片实验室系统的小型化以提供即时医疗诊断具有吸引力。为了进一步评估Fe3O4 MNPs在增强LSPR测定中的实际应用，使用用于心肌梗死诊断的心肌肌钙蛋白I（cTnI）作为模型蛋白，通过金纳米棒（GNR）生物探针进行检测。 MNP 捕获的 cTnI 分子产生的光谱响应比 GNR 传感器上直接吸附的 cTnI 高 6 倍。检测限 (LOD) 降低至约。血浆样本为 30 pM，比同类研究低 3 个数量级。据我们所知，这标志着基于无标记 LSPR 位移且无需复杂仪器的真实血浆蛋白检测的最低 LOD。 Fe3O4 MNP 观察到的 LSPR 传感增强与非特异性结合无关。
We demonstrate that Fe3O4 magnetic nanoparticle (MNP) can greatly enhance the localized surface plasmon resonance (LSPR) of metal nanoparticle. The high refractive index and molecular weight of the Fe3O4 MNPs make them a powerful enhancer for plasmonic response to biological binding events, thereby enabling a significant improvement in the sensitivity, reliability, dynamic range, and calibration linearity for LSPR assay of small molecules in trace amount. Rather than using fluorescence spectroscopy or magnetic resonance imaging, this study marks the first use of the label-free LSPR nanosensor for a disease biomarker in physiological solutions, providing a low cost, clinical-oriented detection. This facile and ultrasensitive nanosensor with extremely light, robust, and low-cost instrument is attractive for miniaturization on a lab-on-a-chip system to deliver point-of-care medical diagnostics. To further evaluate the practical application of Fe3O4 MNPs in the enhancement of LSPR assay, cardiac troponin I (cTnI) for myocardial infarction diagnosis was used as a model protein to be detected by a gold nanorod (GNR) bioprobe. MNP-captured cTnI molecules resulted in spectral responses up to 6 fold higher than direct cTnI adsorption on the GNR sensor. The detection limit (LOD) was lowered to ca. 30 pM for plasma samples which is 3 orders lower than comparable study. To the best of our knowledge, this marks the lowest LOD for a real plasma protein detection based on label-free LSPR shift without complicated instrumentation. The observed LSPR sensing enhancement by Fe3O4 MNPs is independent of nonspecific binding.
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影响因子：
7.4
作者：
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通讯作者：
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