Activation of serum complement by polysaccharide-containing antigens of Porphyromonas gingivalis.
Activation of serum complement by polysaccharide-containing antigens of Porphyromonas gingivalis.
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牙龈卟啉单胞菌含多糖抗原激活血清补体。
DOI：
10.1111/j.1600-0765.1993.tb02091.x
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发表时间：
1993
影响因子：
3.5
通讯作者：
Genco,RJ
中科院分区：
文献类型：
--
作者：
Schifferle,RE;Wilson,ME;Levine,MJ;Genco,RJ
我们以前曾报道过，热酚提取牙龈卟啉单胞菌产生的制剂含有脂多糖（LPS）和抗原性不同的荚膜多糖（PS）。在本研究中，我们研究了一些菌株的苯酚-水提取物的能力。gingivalisto激活人血清补体。抗补体活性的提取物从两个侵入性和两个非侵入性菌株的P。在绵羊红细胞溶血试验和替代途径选择性兔红细胞溶血试验中评估牙龈炎。在绵羊红细胞测定中，发现非侵袭性菌株的提取物比侵袭性菌株的提取物表现出更大的抗互补活性。将侵袭性菌株ATCC 53977的苯酚-水提取物进一步分解成其LPS和PS级分。而从该菌株分离的LPS表现出较强的抗互补活性，PS馏分只有弱活性。发现来自四种菌株中的三种的苯酚-水提取物是旁路途径的有效激活剂，其中来自两种非侵入性菌株的提取物最具活性。剩余菌株（ATCC 53977）的提取物是旁路途径的弱激活剂。然而，对该提取物的进一步分析显示，LPS组分是旁路途径的有效激活剂，尽管PS组分表现出可忽略的活性。这项研究的结果表明，苯酚水提取物的侵入性和非侵入性菌株的P。牙龈炎在它们各自的抗补体活性上不同，侵袭性菌株的活性较低。虽然从侵入性和非侵入性菌株提取物激活的替代途径，这种活动似乎是由于LPS，而不是PS，组件。
We previosly reported that hot aqueous phenol extraction ofPorphyromonas gingivalisyields a preparation containing both lipopolysaccharide (LPS) and an antigenically distinct capsular polysaccharide (PS). In the present study, we examined the capacity of phenol‐water extracts from a number of strains ofP. gingivalisto activate human serum complement. Anticomplementary activity of extracts from two invasive and two noninvasive strains ofP. gingivaliswas assessed in a sheep erythrocyte hemolytic assay and in an alternative pathway‐selective rabbit erythrocyte hemolytic assay. In the sheep erythrocyte assay, extracts from noninvasive strains were found to exhibit greater anticomplementary activity than extracts derived from invasive strains. A phenol‐water extract from invasive strain ATCC 53977 was further resolved into its LPS and PS fractions. Whereas isolated LPS from this strain exhibited strong anticomplementary activity, the PS fraction was only weakly active. Phenol‐water extracts from three of four strains were found to be potent activators of the alternative pathway, with extracts from the two noninvasive strains being most active. The extract from the remaining strain (ATCC 53977) was a poor activator of the alternative pathway. Further analysis of this extract revealed, however, that the LPS fraction was a potent activator of the alternative pathway, although the PS fraction exhibited negligible activity. The results of this study indicate that phenol‐water extracts of invasive and noninvasive strains ofP. gingivalisdiffer in their respective anticomplementary activities, with invasive strains being less active. Although extracts from both invasive and noninvasive strains activated the alternative pathway, this activity appears to be attributable to the LPS, rather than the PS, component.
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影响因子：
3.1
作者：
GREENBLATT, J;BOACKLE, RJ;SCHWAB, JH
通讯作者：
SCHWAB, JH
牙龈拟杆菌与纯化的 C5 相互作用后产生中性粒细胞趋化因子。
影响因子：
3.5
作者：
Schenkein,HA;Berry,CR
通讯作者：
Berry,CR
两种脆弱拟杆菌参考菌株的荚膜多糖和脂多糖：化学和免疫化学表征
影响因子：
3.2
作者：
D. Kasper;A. Weintraub;A. Lindberg;J. Lönngren
通讯作者：
J. Lönngren
牙龈拟杆菌致病菌株对豚鼠补体 C3 蛋白的体内降解。
DOI：
10.1111/j.1600-0722.1984.tb00854.x
发表时间：
1984
期刊：
Scandinavian journal of dental research
影响因子：
--
作者：
G. Sundqvist;J. Carlsson;B. Herrmann;J. F. Höfling;A. Väätäinen
通讯作者：
A. Väätäinen
补体在牙周疾病中的作用。
DOI：
10.1177/10454411910020010501
发表时间：
1991
期刊：
Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists
影响因子：
--
作者：
Schenkein,HA
通讯作者：
Schenkein,HA