Sox10-Venus mice: a new tool for real-time labeling of neural crest lineage cells and oligodendrocytes.
Sox10-Venus mice: a new tool for real-time labeling of neural crest lineage cells and oligodendrocytes.
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DOI：
10.1186/1756-6606-3-31
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发表时间：
2010-10-31
期刊：
影响因子：
3.6
通讯作者：
Okano H
中科院分区：
文献类型：
--
作者：
Shibata S;Yasuda A;Renault-Mihara F;Suyama S;Katoh H;Inoue T;Inoue YU;Nagoshi N;Sato M;Nakamura M;Akazawa C;Okano H
虽然最近开发了几种小鼠品系用于追踪神经嵴或少突胶质细胞谱系，但每种品系都有其固有的局限性。人类 SOX10 突变与神经嵴细胞发病机制之间的联系使我们关注对神经嵴发育至关重要的 Sox10 基因。我们生成了 Sox10-Venus BAC 转基因小鼠来监测正常发育和病理过程中的 Sox10 表达。组织荧光可区分 Sox10-Venus 小鼠胚胎中的神经嵴后代细胞和少突胶质细胞。免疫组织化学分析证实，Venus 表达仅限于表达内源性 Sox10 的细胞。 Sox10-Venus 小鼠各种组织的延时成像表明，由于强烈、聚焦的金星荧光，可以在体内单细胞水平上可视化金星表达。在成年 Sox10-Venus 小鼠中，在脊髓损伤之前和之后，几种类型的成熟和未成熟少突胶质细胞以及雪旺细胞都被清晰地标记为 Venus。在新开发的 Sox10-Venus 转基因小鼠中，Venus 荧光忠实地反映了内源性 Sox10 表达，并允许在单细胞水平上对活细胞进行体内成像。因此，这种 Sox10-Venus 小鼠将成为研究神经嵴细胞或少突胶质细胞发育和病理过程的有用工具。
While several mouse strains have recently been developed for tracing neural crest or oligodendrocyte lineages, each strain has inherent limitations. The connection between human SOX10 mutations and neural crest cell pathogenesis led us to focus on the Sox10 gene, which is critical for neural crest development. We generated Sox10-Venus BAC transgenic mice to monitor Sox10 expression in both normal development and in pathological processes. Tissue fluorescence distinguished neural crest progeny cells and oligodendrocytes in the Sox10-Venus mouse embryo. Immunohistochemical analysis confirmed that Venus expression was restricted to cells expressing endogenous Sox10. Time-lapse imaging of various tissues in Sox10-Venus mice demonstrated that Venus expression could be visualized at the single-cell level in vivo due to the intense, focused Venus fluorescence. In the adult Sox10-Venus mouse, several types of mature and immature oligodendrocytes along with Schwann cells were clearly labeled with Venus, both before and after spinal cord injury. In the newly-developed Sox10-Venus transgenic mouse, Venus fluorescence faithfully mirrors endogenous Sox10 expression and allows for in vivo imaging of live cells at the single-cell level. This Sox10-Venus mouse will thus be a useful tool for studying neural crest cells or oligodendrocytes, both in development and in pathological processes.
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影响因子：
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