Emergence of FRESH 3D printing as a platform for advanced tissue biofabrication.
Emergence of FRESH 3D printing as a platform for advanced tissue biofabrication.
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DOI：
10.1063/5.0032777
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发表时间：
2021-03
影响因子：
6
通讯作者：
Feinberg AW
中科院分区：
文献类型：
--
作者：
Shiwarski DJ;Hudson AR;Tashman JW;Feinberg AW
在组织工程中，一个尚未解决的挑战是如何构建复杂的3D支架，以重建人体组织和器官的结构和功能。3D生物打印等添加剂制造技术有可能构建具有前所未有的空间控制的生物材料；然而，在空气中打印软生物材料往往会导致保真度较低。自由形式可逆嵌入悬浮水凝胶(FRESH)是一种嵌入式打印方法，通过在屈服应力支撑浴液中挤出生物油墨来解决这一问题，该浴液将生物油墨保持在适当的位置直到固化。在这个视角下，我们讨论了3D打印软性和液体状生物墨水的挑战，以及作为解决方案的新的和相关的嵌入式打印技术的出现。这包括生物打印领域新的嵌入式3D打印的发展和采用的快速增长，以及新打印用于生物制造的优势和由此带来的新的研究成果。特别关注的是新打印技术的可定制性，其中屈服应力支撑液和水相交联剂的化学成分都可以为打印复杂3D结构的各种生物墨水量身定做。最后，我们展望了新鲜印刷的未来，讨论了随着生物制造领域的发展，我们看到的挑战和机遇。
In tissue engineering, an unresolved challenge is how to build complex 3D scaffolds in order to recreate the structure and function of human tissues and organs. Additive manufacturing techniques, such as 3D bioprinting, have the potential to build biological material with unprecedented spatial control; however, printing soft biological materials in air often results in poor fidelity. Freeform Reversible Embedding of Suspended Hydrogels (FRESH) is an embedded printing approach that solves this problem by extruding bioinks within a yield-stress support bath that holds the bioinks in place until cured. In this Perspective, we discuss the challenges of 3D printing soft and liquid-like bioinks and the emergence for FRESH and related embedded printing techniques as a solution. This includes the development of FRESH and embedded 3D printing within the bioprinting field and the rapid growth in adoption, as well as the advantages of FRESH printing for biofabrication and the new research results this has enabled. Specific focus is on the customizability of the FRESH printing technique where the chemical composition of the yield-stress support bath and aqueous phase crosslinker can all be tailored for printing a wide range of bioinks in complex 3D structures. Finally, we look ahead at the future of FRESH printing, discussing both the challenges and the opportunities that we see as the biofabrication field develops.
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影响因子：
9
作者：
Adib, A. Asghari;Sheikhi, A.;Hoelzle, D. J.
通讯作者：
Hoelzle, D. J.
影响因子：
4.6
作者：
Afghah, Ferdows;Altunbek, Mine;Koc, Bahattin
通讯作者：
Koc, Bahattin
影响因子：
9
作者：
Campos, Daniela F. Duarte;Blaeser, Andreas;Fischer, Horst
通讯作者：
Fischer, Horst
DOI：
10.1126/science.aav9750
发表时间：
2019-05-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Grigoryan B;Paulsen SJ;Corbett DC;Sazer DW;Fortin CL;Zaita AJ;Greenfield PT;Calafat NJ;Gounley JP;Ta AH;Johansson F;Randles A;Rosenkrantz JE;Louis-Rosenberg JD;Galie PA;Stevens KR;Miller JS
通讯作者：
Miller JS
影响因子：
13.6
作者：
Bhattacharjee T;Zehnder SM;Rowe KG;Jain S;Nixon RM;Sawyer WG;Angelini TE
通讯作者：
Angelini TE