A Pilot Study on MicroRNA Profile in Tear Fluid to Predict Response to Anti-VEGF Treatments for Diabetic Macular Edema.
A Pilot Study on MicroRNA Profile in Tear Fluid to Predict Response to Anti-VEGF Treatments for Diabetic Macular Edema.
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DOI：
10.3390/jcm9092920
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发表时间：
2020-09-10
影响因子：
3.9
通讯作者：
Su X
中科院分区：
文献类型：
--
作者：
Chan HW;Yang B;Wong W;Blakeley P;Seah I;Tan QSW;Wang H;Bhargava M;Lin HA;Chai CH;Mangunkusumo EA;Thet N;Yuen YS;Sethi R;Wang S;Hunziker W;Lingam G;Su X
(1)背景：玻璃体内注射抗血管内皮生长因子(anti-VEGF)是治疗中心性糖尿病黄斑水肿(ci-DME)的有效方法。然而，临床反应是不同的。本研究探讨了miRNAs作为预测DME中抗血管内皮生长因子治疗反应的生物标志物。(2)方法：收集初治DME患者的泪液、房水和血液，用定量聚合酶链式反应分析miRNA的表达。从泪液中鉴定出反应良好和反应差的患者之间差异表达的miRNAs。利用miEAA工具、miRTarBase注释、基因本体论类别、KEGG和miRWalk路径进行的生物信息学分析确定了丰富的miRNAs与生物路径之间的相互作用。(3)结果：24例患者中，贝伐单抗治疗15例(28眼)，阿普利赛特治疗13例(13眼)。泪液的miRNA种类最多(N=315)，其次是血清(N=309)，然后是房水(N=134)。与黄斑厚度变化相关的miRNAs在反应良好的患者中为miR-214-3p、miR-320d和hsa-miR-874-3p，在反应不良的患者中为miR-98-5p、miR-196b-5p和miR-454-3p。血管内皮生长因子相关途径和血管生成素-PrI复合体在反应良好的患者中丰富，而转化生长因子-β和胰岛素样生长因子途径在反应不良的患者中丰富。(4)结论：我们报道了一组新的miRNAs，这些miRNAs为深入了解DME的生物学途径提供了线索。需要在更大的独立队列中进行验证，以确定这些miRNA候选生物标记物的预测性能。
(1) Background: Intravitreal anti-vascular endothelial growth factor (anti-VEGF) is an established treatment for center-involving diabetic macular edema (ci-DME). However, the clinical response is heterogeneous. This study investigated miRNAs as a biomarker to predict treatment response to anti-VEGF in DME. (2) Methods: Tear fluid, aqueous, and blood were collected from patients with treatment-naïve DME for miRNA expression profiling with quantitative polymerase chain reaction. Differentially expressed miRNAs between good and poor responders were identified from tear fluid. Bioinformatics analysis with the miEAA tool, miRTarBase Annotations, Gene Ontology categories, KEGG, and miRWalk pathways identified interactions between enriched miRNAs and biological pathways. (3) Results: Of 24 participants, 28 eyes received bevacizumab (15 eyes) or aflibercept (13 eyes). Tear fluid had the most detectable miRNA species (N = 315), followed by serum (N = 309), then aqueous humor (N = 134). MiRNAs that correlated with change in macular thickness were miR-214-3p, miR-320d, and hsa-miR-874-3p in good responders; and miR-98-5p, miR-196b-5p, and miR-454-3p in poor responders. VEGF-related pathways and the angiogenin-PRI complex were enriched in good responders, while transforming growth factor-β and insulin-like growth factor pathways were enriched in poor responders. (4) Conclusions: We reported a panel of novel miRNAs that provide insight into biological pathways in DME. Validation in larger independent cohorts is needed to determine the predictive performance of these miRNA candidate biomarkers.
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影响因子：
10.8
作者：
Bates DO
通讯作者：
Bates DO
抗血管内皮生长因子比较有效性试验的糖尿病性黄斑水肿：一项随机临床试验的事后分析后的其他功效。
影响因子：
8.1
作者：
Jampol LM;Glassman AR;Bressler NM;Wells JA;Ayala AR;Diabetic Retinopathy Clinical Research Network
通讯作者：
Diabetic Retinopathy Clinical Research Network
影响因子：
14.9
作者：
The Gene Ontology Consortium
通讯作者：
The Gene Ontology Consortium
影响因子：
7.7
作者：
Feng B;Chen S;McArthur K;Wu Y;Sen S;Ding Q;Feldman RD;Chakrabarti S
通讯作者：
Chakrabarti S
影响因子：
4.4
作者：
Harhaj, Nicole S.;Felinski, Edward A.;Antonetti, David A.
通讯作者：
Antonetti, David A.