Short-term integration of Cdc25 dynamics controls mitotic entry during Drosophila gastrulation.
Short-term integration of Cdc25 dynamics controls mitotic entry during Drosophila gastrulation.
复制标题
DOI：
10.1016/j.devcel.2012.01.019
复制
发表时间：
2012-04-17
影响因子：
11.8
通讯作者：
Wieschaus, Eric F.
中科院分区：
文献类型：
--
作者：
Di Talia, Stefano;Wieschaus, Eric F.
细胞通过突然激活有丝分裂的细胞周期蛋白-CDK复合体进行有丝分裂。在果蝇原肠发育过程中，有丝分裂与cdc25串的转录激活有关，cdc25串是一种激活cdk1的磷酸酶。在这里，我们证明了在果蝇胚胎中观察到的第14个有丝分裂周期中的开关样进入有丝分裂是由Cdc25串积累的动力学决定的。该开关作为短期积分器运行，这一特性可以提高对有丝分裂时间的可靠控制。该开关与CDK1和CDC25STRING之间的正反馈以及CDK1和Wee1之间的双重负反馈无关。我们认为，有丝分裂开关的性质是由控制CDK1活性的共价修饰循环的非平衡性质建立的。这种由激活酶转录表达触发的共价修饰循环，可能是一种广泛使用的策略，以获得对细胞决策的可靠和开关样的控制。
Cells commit to mitosis by abruptly activating the mitotic cyclin-Cdk complexes. During Drosophila gastrulation, mitosis is associated with the transcriptional activation of cdc25string, a phosphatase that activates Cdk1. Here, we demonstrate that the switch-like entry into mitosis observed in the Drosophila embryo during the 14th mitotic cycle is timed by the dynamics of Cdc25string accumulation. The switch operates as a short-term integrator, a property that can improve the reliable control of timing of mitosis. The switch is independent of the positive feedback between Cdk1 and Cdc25string and of the double negative feedback between Cdk1 and Wee1. We propose that the properties of the mitotic switch are established by the out-of-equilibrium properties of the covalent modification cycle controlling Cdk1 activity. Such covalent modification cycles, triggered by transcriptional expression of the activating enzymes, might be a widespread strategy to obtain reliable and switch-like control of cell decisions.
登录
查看更多内容
影响因子：
4.3
作者：
Gomez-Uribe C;Verghese GC;Mirny LA
通讯作者：
Mirny LA
影响因子：
64.5
作者：
Harvey, SL;Charlet, A;Kellogg, DR
通讯作者：
Kellogg, DR
影响因子：
64.5
作者：
KUMAGAI, A;DUNPHY, WG
通讯作者：
DUNPHY, WG
影响因子：
64.5
作者：
EDGAR, BA;OFARRELL, PH
通讯作者：
OFARRELL, PH
DOI：
10.1073/pnas.78.11.6840
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GOLDBETER, A;KOSHLAND, DE
通讯作者：
KOSHLAND, DE