Abscisic acid-induced cytoplasmic translocation of constitutive photomorphogenic 1 enhances reactive oxygen species accumulation through the HY5-ABI5 pathway to modulate seed germination.

Abscisic acid-induced cytoplasmic translocation of constitutive photomorphogenic 1 enhances reactive oxygen species accumulation through the HY5-ABI5 pathway to modulate seed germination.
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DOI:
10.1111/pce.14298
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发表时间:
2022-05
影响因子:
7.3
通讯作者:
Song, Chun-Peng
Song, Chun-Peng
中科院分区:
生物学1区
文献类型:
--
作者:
Chen, Qing-Bin;Wang, Wen-Jing;Zhang, Yue;Zhan, Qi-Di;Liu, Kang;Botella, Jose Ramon;Bai, Ling;Song, Chun-Peng

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种子萌发是一个受多种因素调控的生理过程。脱落酸(ABA)可以抑制种子萌发,提高幼苗在非生物胁迫条件下的存活率,这一过程通常受到光信号的调控。组成型光形态发生 1 (COP1) 是光信号的上游核心抑制因子,参与多种 ABA 反应。在这里,我们证明 COP1 是 ABA 介导的种子萌发抑制的负调节因子。 COP1 的破坏增强了拟南芥种子对 ABA 的敏感性并增加了活性氧 (ROS) 水平。在种子中,ABA 诱导 COP1 易位到细胞质,导致 ABA 诱导的 ROS 水平增强。遗传证据表明,HY5 和 ABI5 在 ABA 介导的种子萌发抑制中作用于 COP1 下游。 ABA 诱导的 COP1 细胞质定位增加了细胞核中 HY5 和 ABI5 蛋白的水平,导致种子中 ABI5 靶基因的表达和 ROS 水平增加。总之,我们的结果表明,ABA 诱导的 COP1 细胞质易位激活 HY5-ABI5 通路,促进 ABA 响应基因的表达和 ABA 介导的种子萌发抑制过程中 ROS 的积累。这些发现增强了 COP1 在 ABA 信号转导途径中的作用。
Seed germination is a physiological process regulated by multiple factors. Abscisic acid (ABA) can inhibit seed germination to improve seedling survival under conditions of abiotic stress, and this process is often regulated by light signals. Constitutive photomorphogenic 1 (COP1) is an upstream core repressor of light signals and is involved in several ABA responses. Here, we demonstrate that COP1 is a negative regulator of the ABA‐mediated inhibition of seed germination. Disruption of COP1 enhanced Arabidopsis seed sensitivity to ABA and increased reactive oxygen species (ROS) levels. In seeds, ABA induced the translocation of COP1 to the cytoplasm, resulting in enhanced ABA‐induced ROS levels. Genetic evidence indicated that HY5 and ABI5 act downstream of COP1 in the ABA‐mediated inhibition of seed germination. ABA‐induced COP1 cytoplasmic localization increased HY5 and ABI5 protein levels in the nucleus, leading to increased expression of ABI5 target genes and ROS levels in seeds. Together, our results reveal that ABA‐induced cytoplasmic translocation of COP1 activates the HY5‐ABI5 pathway to promote the expression of ABA‐responsive genes and the accumulation of ROS during ABA‐mediated inhibition of seed germination. These findings enhance the role of COP1 in the ABA signal transduction pathway.
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