Expression of an Antiviral Gene GmRUN1 from Soybean Is Regulated via Intron-Mediated Enhancement (IME).
Expression of an Antiviral Gene GmRUN1 from Soybean Is Regulated via Intron-Mediated Enhancement (IME).
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大豆抗病毒基因 GmRUN1 的表达通过内含子介导的增强 (IME) 进行调节
DOI：
10.3390/v13102032
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发表时间：
2021-10-08
期刊：
影响因子：
--
通讯作者：
Wuriyanghan H
中科院分区：
文献类型：
--
作者：
Diao P;Sun H;Bao Z;Li W;Niu N;Li W;Wuriyanghan H
大多数抗病基因编码含有NBS-LRR结构域的蛋白质。给你N在3-4周龄时使用本塞姆氏植物进行瞬时表达测定。我们鉴定了一个TNL（TIR-NBS-LRR）编码基因GmRUN 1，该基因对大豆花叶病毒（SMV）和烟草花叶病毒（TMV）均具有抗性。截断分析表明所有三个典型结构域对GmRUN 1介导的抗病毒活性的重要性。启动子GUS分析表明，GmRUN 1的表达是诱导水杨酸（SA）和转录因子GmDREB 3通过顺式元件作为-1和ERE（乙烯反应元件），这是目前在其启动子区域。有趣的是，GmRUN 1 gDNA（基因组DNA）显示出比其cDNA（互补DNA）更高的病毒抗性，表明GmRUN 1调控存在内含子介导的增强（IME）。我们提供的证据表明，GmRUN 1的内含子2增加了天然基因GmRUN 1，大豆抗病毒基因SRC 7和报告基因荧光素酶的mRNA水平，表明内含子2在不同基因中的普遍转录增强。综上所述，我们鉴定了一个抗TNL型大豆基因GmRUN 1，其表达在不同层中受到调控。对GmRUN 1基因调控网络的研究有助于探索大豆与SMV互作的机制。
Most of R (resistance) genes encode the protein containing NBS-LRR (nucleotide binding site and leucine-rich repeat) domains. Here, N. benthamiana plants were used for transient expression assays at 3–4 weeks of age. We identified a TNL (TIR-NBS-LRR) encoding gene GmRUN1 that was resistant to both soybean mosaic virus (SMV) and tobacco mosaic virus (TMV). Truncation analysis indicated the importance of all three canonical domains for GmRUN1-mediated antiviral activity. Promoter-GUS analysis showed that GmRUN1 expression is inducible by both salicylic acid (SA) and a transcription factor GmDREB3 via the cis-elements as-1 and ERE (ethylene response element), which are present in its promoter region. Interestingly, GmRUN1 gDNA (genomic DNA) shows higher viral resistance than its cDNA (complementary DNA), indicating the existence of intron-mediated enhancement (IME) for GmRUN1 regulation. We provided evidence that intron2 of GmRUN1 increased the mRNA level of native gene GmRUN1, a soybean antiviral gene SRC7 and also a reporter gene Luciferase, indicating the general transcriptional enhancement of intron2 in different genes. In summary, we identified an antiviral TNL type soybean gene GmRUN1, expression of which was regulated at different layers. The investigation of GmRUN1 gene regulatory network would help to explore the mechanism underlying soybean-SMV interactions.
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影响因子：
3.5
作者：
He, LM;Du, CG;Zhang, HB
通讯作者：
Zhang, HB
影响因子：
11.6
作者：
Bieri, S;Mauch, S;Schulze-Lefert, P
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Schulze-Lefert, P
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10.5
作者：
CALLIS, J;FROMM, M;WALBOT, V
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WALBOT, V
影响因子：
4.3
作者：
Liao, Liao;Ning, Guogui;Bao, Manzhu
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Bao, Manzhu
影响因子：
7.4
作者：
Garretón, V;Carpinelli, J;Holuigue, L
通讯作者：
Holuigue, L