Difficulties in Differentiating Coronaviruses from Subcellular Structures in Human Tissues by Electron Microscopy.
Difficulties in Differentiating Coronaviruses from Subcellular Structures in Human Tissues by Electron Microscopy.
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DOI：
10.3201/eid2704.204337
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发表时间：
2021-04
影响因子：
11.8
通讯作者：
Miller SE
中科院分区：
文献类型：
--
作者：
Bullock HA;Goldsmith CS;Zaki SR;Martines RB;Miller SE
在抗击由严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）引起的冠状病毒病（COVID-19）大流行的努力中，人们再次关注使用透射电子显微镜识别组织中的冠状病毒。在试图将COVID-19患者的病理直接归因于SARS-CoV-2引起的组织损伤时，研究人员错误地将亚细胞结构（包括包被囊泡、多泡体和囊泡粗内质网）报告为冠状病毒颗粒。我们描述了冠状病毒与亚细胞结构的形态学特征，包括颗粒大小范围（60-140 nm），细胞内颗粒在膜结合液泡内的位置，以及核衣壳在颗粒内的密集点（6-12 nm）出现在横截面上。此外，尽管在病毒表面，特别是在细胞外颗粒上可以看到冠状病毒的特征尖峰，但在薄片上比在阴性染色制剂中更不明显。
Efforts to combat the coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have placed a renewed focus on the use of transmission electron microscopy for identifying coronavirus in tissues. In attempts to attribute pathology of COVID-19 patients directly to tissue damage caused by SARS-CoV-2, investigators have inaccurately reported subcellular structures, including coated vesicles, multivesicular bodies, and vesiculating rough endoplasmic reticulum, as coronavirus particles. We describe morphologic features of coronavirus that distinguish it from subcellular structures, including particle size range (60–140 nm), intracellular particle location within membrane-bound vacuoles, and a nucleocapsid appearing in cross section as dense dots (6–12 nm) within the particles. In addition, although the characteristic spikes of coronaviruses may be visible on the virus surface, especially on extracellular particles, they are less evident in thin sections than in negative stain preparations.
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