Uniformly sized molecularly imprinted polymers for on-line concentration, purification, and measurement of nimodipine in plasma
Uniformly sized molecularly imprinted polymers for on-line concentration, purification, and measurement of nimodipine in plasma
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用于在线浓缩、纯化和测量血浆中尼莫地平的均匀尺寸的分子印迹聚合物
DOI：
10.1002/app.29315
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发表时间：
2009-03
影响因子：
3
通讯作者：
Qi Fang
中科院分区：
文献类型：
--
作者：
Qianqian Zhang;Chun Chang;Langchong He;Elijan Amut;Qiang Fu;Qi Fang
以4-乙烯基吡啶（4-VPY）或甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EDMA）为交联剂，采用多步溶胀聚合法，在水溶液中制备了尺寸均匀的尼莫地平分子印迹聚合物（MIPs）。用扫描电子显微镜对所得聚合物的结构特征进行了表征。进一步考察了色谱条件的影响，探讨了可能的识别机理。结果表明，所制备的分子印迹聚合物微球稳定、粒径单分散性好，对模板分子和二氢吡啶类钙拮抗剂具有特异性识别。除了疏水相互作用外，DHP和MIPs的分子形状互补似乎在DHP的保留和识别中起着重要作用。Scatchard分析表明，分子印迹聚合物中存在两种结合位点。然后将MIPs用作高效液相色谱（HPLC）分离介质，以同时浓缩和纯化血浆中的尼莫地平。结果表明，所制备的分子印迹聚合物可用于生物样品中尼莫地平的在线富集、纯化和测定。© 2008 Wiley Periodicals，Inc.应用聚合物科学杂志，2009年
Uniformly sized molecularly imprinted polymers (MIPs) for nimodipine have been prepared in an aqueous system by multi-step swelling and polymerization method, utilizing 4-vinylpyridine (4-VPY) or methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EDMA) as a cross-linking agent. Scanning electron microscopy was used to identify the structure features of the obtained polymers. Further, the influences of some chromatographic conditions were examined to explore the possible recognition mechanism. The results reveal that stable molecularly imprinted polymeric microspheres with good size monodispersity were obtained, and the polymer beads showed specific recognition for the template molecule and some other dihydropyridine calcium antagonists (DHPs). Besides hydrophobic interaction, the molecular shape complementation of DHPs and the MIPs seems to play an important role in the retention and recognition of DHPs. The Scatchard analysis showed that two kinds of binding sites existed in the MIPs. The MIPs was then used as a high-performance liquid chromatography (HPLC) separation medium to simultaneously concentrate and purify nimodipine in plasma. The results reveal that the obtained MIPs could be used for on-line concentration, purification, and measurement of nimodipine in biological samples. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2009
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影响因子：
7.4
作者：
V. Remcho;Z. Tan
通讯作者：
V. Remcho;Z. Tan
DOI：
10.1039/a806379f
发表时间：
1998
期刊：
Analytical Communications
影响因子：
--
作者：
J. Olsen;Paul D. Martin;I. Wilson
通讯作者：
J. Olsen;Paul D. Martin;I. Wilson
DOI：
10.1039/b202295h
发表时间：
2002-10
期刊：
The Analyst
影响因子：
--
作者：
X. Dong;Hui Sun;Xianyu Lu;Haibo Wang;Shuxia Liua;Ning Wang
通讯作者：
X. Dong;Hui Sun;Xianyu Lu;Haibo Wang;Shuxia Liua;Ning Wang
影响因子：
4.1
作者：
Haginaka, J;Takekira, H;Tanaka, N
通讯作者：
Tanaka, N
DOI：
10.1039/a700103g
发表时间：
1997-03-01
期刊：
ANALYTICAL COMMUNICATIONS
影响因子：
--
作者：
Matsui, J;Okada, M;Takeuchi, T
通讯作者：
Takeuchi, T