A taxonomy of transcriptomic cell types across the isocortex and hippocampal formation.
A taxonomy of transcriptomic cell types across the isocortex and hippocampal formation.
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DOI：
10.1016/j.cell.2021.04.021
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发表时间：
2021-06-10
期刊：
影响因子：
64.5
通讯作者：
Zeng H
中科院分区：
文献类型：
--
作者：
Yao Z;van Velthoven CTJ;Nguyen TN;Goldy J;Sedeno-Cortes AE;Baftizadeh F;Bertagnolli D;Casper T;Chiang M;Crichton K;Ding SL;Fong O;Garren E;Glandon A;Gouwens NW;Gray J;Graybuck LT;Hawrylycz MJ;Hirschstein D;Kroll M;Lathia K;Lee C;Levi B;McMillen D;Mok S;Pham T;Ren Q;Rimorin C;Shapovalova N;Sulc J;Sunkin SM;Tieu M;Torkelson A;Tung H;Ward K;Dee N;Smith KA;Tasic B;Zeng H
哺乳动物大脑中的同皮层和海马结构（HPF）在感知、认知、情感和学习中起着关键作用。我们分析了约130万个覆盖整个成年小鼠同皮质和HPF的细胞，并推导出一个转录组细胞类型分类法，揭示了一个全面的多巴胺能和GABA能神经元类型的库。与HPF具有更简单的细胞组织的传统观点相反，我们发现了一套完整的HPF中与六层同皮质中发现的所有主要亚类同源的神经元类型，这表明HPF和同皮质共享一个共同的电路组织。我们还确定了大规模的连续和分级变化的细胞类型沿着等皮层深度，跨等皮层片和在多个维度的海马和下托。总的来说，我们的研究建立了哺乳动物同皮质和海马结构的分子结构，并开始揭示其与这些大脑结构的发育，进化，连接和功能的潜在关系。整个小鼠同皮质和海马结构的单细胞转录组学显示了共享的细胞和电路组织以及大规模的神经元类型变化的连续梯度，这些变化阐明了这两个关键脑结构之间的潜在关系。
The isocortex and hippocampal formation (HPF) in the mammalian brain play critical roles in perception, cognition, emotion and learning. We profiled ~1.3 million cells covering the entire adult mouse isocortex and HPF and derived a transcriptomic cell type taxonomy revealing a comprehensive repertoire of glutamatergic and GABAergic neuron types. Contrary to the traditional view of HPF as having a simpler cellular organization, we discover a complete set of glutamatergic types in HPF homologous to all major subclasses found in the six-layered isocortex, suggesting that HPF and isocortex share a common circuit organization. We also identify large-scale continuous and graded variation of cell types along isocortical depth, across isocortical sheet and in multiple dimensions in hippocampus and subiculum. Overall, our study establishes a molecular architecture of mammalian isocortex and hippocampal formation and begins to shed light on its underlying relationship with the development, evolution, connectivity and function of these brain structures. Single-cell transcriptomics of entire mouse isocortex and hippocampal formation shows shared cellular and circuit organization and large-scale continuous gradients of neuron type variation that illuminates the underlying relationship between these two critical brain structures.
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影响因子：
7.7
作者：
Cembrowski MS;Wang L;Lemire AL;Copeland M;DiLisio SF;Clements J;Spruston N
通讯作者：
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影响因子：
16.2
作者：
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通讯作者：
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影响因子：
64.8
作者：
Harris JA;Mihalas S;Hirokawa KE;Whitesell JD;Choi H;Bernard A;Bohn P;Caldejon S;Casal L;Cho A;Feiner A;Feng D;Gaudreault N;Gerfen CR;Graddis N;Groblewski PA;Henry AM;Ho A;Howard R;Knox JE;Kuan L;Kuang X;Lecoq J;Lesnar P;Li Y;Luviano J;McConoughey S;Mortrud MT;Naeemi M;Ng L;Oh SW;Ouellette B;Shen E;Sorensen SA;Wakeman W;Wang Q;Wang Y;Williford A;Phillips JW;Jones AR;Koch C;Zeng H
通讯作者：
Zeng H
影响因子：
13.9
作者：
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通讯作者：
Rudy B