Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques

Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques
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使用新的水族箱技术以及混合光学显微镜和光学相干断层扫描(OCT)成像技术揭示活磷虾的内部生理学

DOI:
10.1080/10236244.2015.1073455
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发表时间:
2015
影响因子:
1
通讯作者:
Cox M
Cox M
中科院分区:
生物学4区
文献类型:
--
作者:
Cox M

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对南极磷虾等重要动物活体样品的生理变化进行精确观察是帮助理解环境变化如何影响动物发育的重要目标。使用定制的“磷虾陷阱”,活的未麻醉的磷虾被限制了七个小时,在此期间,获得了三个小时的光学成像,没有观察到随后的不良影响。该陷阱使两种成像方法被采用:光学相干断层扫描(OCT)和显微镜。OCT能够对内部结构和组织进行成像,深度约为2 mm,分辨率约为12 μm。光镜下观察心率。在我们的实验中,我们对活体动物的一系列内部结构进行了成像,包括心脏和胃区域。陷阱的设计,使新一代的混合模态成像这些动物在体内。这些技术将使活磷虾的内部生理学的详细研究在广泛的环境条件下进行,并有可能突出行为和动物发育的重要变化。
The accurate observation of physiological changes onin vivosamples of important animal species such asEuphausia superba(Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized krill were confined for seven hours, during which three hours of optical imaging were obtained and no subsequent ill effects observed. The trap enabled two imaging methods to be employed: optical coherence tomography (OCT) and microscopy. OCT enabled internal structure and tissues to be imaged to a depth of approximately 2 mm and resolution of approximately 12 μm. Microscopy was used to observe heart rate. During our experiments, we imaged a range of internal structures in live animals including the heart and gastric areas. The trap design enables a new generation of mixed modality imaging of these animalsin vivo. These techniques will enable detailed studies of the internal physiology of live krill to be undertaken under a wide range of environmental conditions and have the potential to highlight important variations in behaviour and animal development.
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