Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques
Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques
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使用新的水族箱技术以及混合光学显微镜和光学相干断层扫描(OCT)成像技术揭示活磷虾的内部生理学
DOI:
10.1080/10236244.2015.1073455
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发表时间:
2015
影响因子:
1
通讯作者:
Cox M
中科院分区:
文献类型:
--
作者:
Cox M
The accurate observation of physiological changes onin vivosamples of important animal species such asEuphausia superba(Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized krill were confined for seven hours, during which three hours of optical imaging were obtained and no subsequent ill effects observed. The trap enabled two imaging methods to be employed: optical coherence tomography (OCT) and microscopy. OCT enabled internal structure and tissues to be imaged to a depth of approximately 2 mm and resolution of approximately 12 μm. Microscopy was used to observe heart rate. During our experiments, we imaged a range of internal structures in live animals including the heart and gastric areas. The trap design enables a new generation of mixed modality imaging of these animalsin vivo. These techniques will enable detailed studies of the internal physiology of live krill to be undertaken under a wide range of environmental conditions and have the potential to highlight important variations in behaviour and animal development.
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影响因子:
6.7
作者:
S. Fevolden
通讯作者:
S. Fevolden
DOI:
--
发表时间:
2003
期刊:
影响因子:
--
作者:
S. Nicol
通讯作者:
S. Nicol
影响因子:
3.3
作者:
Kawaguchi, So;Kurihara, Haruko;Ishimatsu, Atsushi
通讯作者:
Ishimatsu, Atsushi
DOI:
10.1109/omems.2002.1031485
发表时间:
2002-11
期刊:
IEEE/LEOS International Conference on Optical MEMs
影响因子:
--
作者:
J. Fujimoto
通讯作者:
J. Fujimoto
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
T. Letessier;S. Kawaguchi;R. King;J. Meeuwig;R. Harcourt;M. Cox
通讯作者:
M. Cox