Lactobacillus johnsonii N5 from heat stress-resistant pigs improves gut mucosal immunity and barrier in dextran sodium sulfate-induced colitis.
Lactobacillus johnsonii N5 from heat stress-resistant pigs improves gut mucosal immunity and barrier in dextran sodium sulfate-induced colitis.
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DOI：
10.1016/j.aninu.2023.04.012
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发表时间：
2023-12
期刊：
影响因子：
6.3
通讯作者：
Liu, Hao-Yu
中科院分区：
文献类型：
--
作者：
Yuan, Long;Zhu, Chuyang;Gu, Fang;Zhu, Miaonan;Yao, Jiacheng;Zhu, Cuipeng;Li, Shicheng;Wang, Kun;Hu, Ping;Zhang, Yunzeng;Cai, Demin;Liu, Hao-Yu
发展有效的策略来预防哺乳动物腹泻和相关的肠道疾病具有重要意义。由于肠道微生物群提供的许多健康益处，例如对抗环境干扰，我们探索了宿主表型相关微生物及其益生菌潜力。通过对慢性热应激断奶仔猪表现为热应激敏感（HS-SUS）和热应激抗性（HS-RES）个体的观察，证实了二者在生长性能上的表型差异肠道热休克蛋白70（HSP 70）调节（P <0.01）和炎症反应（P <0.01）。通过16 S rRNA基因测序和KEGG功能分析，发现HS-RES仔猪中约氏乳杆菌的相对丰度显著高于HS-SUS仔猪（P <0. 05）。使用化学诱导的炎症和肠损伤的小鼠模型的进一步实验证明，口服施用代表性的约氏乳杆菌N5（分离自HS-RES仔猪）改善了结肠炎的临床和组织学体征，同时抑制肠促炎细胞因子TNF-α和IL-6的产生（P < 0.05）。结果表明，N5处理后，结肠炎组织中紧密连接蛋白ZO-1和occludin的表达明显增加，细胞保护性蛋白HSP 70的表达也明显增加（P < 0.05），结肠炎组织中紧密连接蛋白ZO-1和occludin的表达明显恢复（P < 0.05）。在生理条件下和结肠炎期间，N5促进肠道派伊尔集合淋巴结MHCII+和CD 103+树突状细胞的数量（P < 0.05），增加调节性T（Treg）细胞的数量（P <0.05），并降低Th 17细胞的数量及其IL-17 α的产生（P < 0.01），这支持了抗炎细胞因子IL-10的高产生（P < 0.01）。我们的研究结果有助于了解嗜酸乳杆菌与宿主健康之间的相互作用，并提供约氏乳杆菌N5作为预防腹泻和肠道疾病的抗生素的替代品。
Developing effective strategies to prevent diarrhea and associated-gut disorders in mammals has gained great significance. Owing to the many health benefits provided by the commensal microbiota of the intestinal tract, such as against environmental perturbation, we explored the host phenotype-associated microbes and their probiotic potential. Based on the observations that the chronic heat stress-exposed weaned piglets present as heat stress-susceptible (HS-SUS) or heat stress-resistant (HS-RES) individuals, we confirmed the phenotypic difference between the two on growth performance (P < 0.05), diarrhea index (P < 0.001), intestinal heat shock protein 70 (HSP70) regulation (P < 0.01), and inflammatory responses (P < 0.01). By comparing the gut microbiome using 16S rRNA gene sequencing and KEGG functional analysis, we found that Lactobacillus johnsonii exhibited significantly higher relative abundance in the HS-RES piglets than in the HS-SUS ones (P < 0.05). Further experiments using a mouse model for chemical-induced inflammation and intestinal injury demonstrated that oral administration of a representative L. johnsonii N5 (isolated from the HS-RES piglets) ameliorated the clinical and histological signs of colitis while suppressing intestinal pro-inflammatory cytokines TNF-α and IL-6 production (P < 0.05). We found that N5 treatment enhanced tight junction proteins ZO-1 and occludin and cytoprotective HSP70 levels under physiological condition and restored their mucosal expressions in colitis (P < 0.05). In support of the high production of the anti-inflammatory cytokine IL-10, N5 promoted the intestinal Peyer's patches MHCII+ and CD103+ dendritic cell populations (P < 0.05), increased the regulatory T (Treg) cell numbers (P < 0.05), and decreased the Th17 population and its IL-17a production under physiological condition and during colitis (P < 0.01). Our results shed light on understanding the interaction between commensal Lactobacillus and the host health, and provide L. johnsonii N5 as an alternative to antibiotics for preventing diarrhea and intestinal diseases.
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影响因子：
3.3
作者：
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