The intestinal archaea Methanosphaera stadtmanae and Methanobrevibacter smithii activate human dendritic cells.
The intestinal archaea Methanosphaera stadtmanae and Methanobrevibacter smithii activate human dendritic cells.
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DOI：
10.1371/journal.pone.0099411
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Schmitz RA
中科院分区：
文献类型：
--
作者：
Bang C;Weidenbach K;Gutsmann T;Heine H;Schmitz RA
已知甲烷古菌Methanoarchaea Methanosphaera stadtmanae和Methanobrevibacter smithii是土著人类肠道微生物群的一部分。尽管细菌肠道菌群的免疫调节作用在过去十年中得到了广泛的研究，但甲烷古菌对人类健康和疾病的影响却很少被研究。因此，我们研究和报告在这里的影响M。stadtmanae和M. Smithii对人体免疫细胞的影响而暴露于M. stadtmanae导致单核细胞来源的树突状细胞（moDC）中大量释放促炎细胞因子，但在与M.史密斯。M.通过共聚焦显微镜以及透射电子显微镜（TEM）证明了moDC的stadtmanae，并且通过使用特异性抑制剂显示出对细胞活化至关重要。尽管程度不同，但两种菌株都启动了moDC中的成熟程序，如细胞表面受体CD86和CD197的上调所揭示的，这表明了适应性免疫应答的额外激活。此外，M。stadtmanae和M. smithii能够在不同程度上改变moDC中抗菌肽的基因表达。综上所述，我们的发现有力地证明了古细菌肠道居民M。stadtmanae和M. Smithii被人类先天免疫系统特异性识别。此外，这两种菌株都能够在不同程度上诱导炎症细胞因子反应，这表明它们可能具有不同的免疫调节功能。总之，我们认为肠道甲烷古菌对涉及肠道微生物群的病理条件的影响到目前为止一直被低估。
The methanoarchaea Methanosphaera stadtmanae and Methanobrevibacter smithii are known to be part of the indigenous human gut microbiota. Although the immunomodulatory effects of bacterial gut commensals have been studied extensively in the last decade, the impact of methanoarchaea in human's health and disease was rarely examined. Consequently, we studied and report here on the effects of M. stadtmanae and M. smithii on human immune cells. Whereas exposure to M. stadtmanae leads to substantial release of proinflammatory cytokines in monocyte-derived dendritic cells (moDCs), only weak activation was detected after incubation with M. smithii. Phagocytosis of M. stadtmanae by moDCs was demonstrated by confocal microscopy as well as transmission electronic microscopy (TEM) and shown to be crucial for cellular activation by using specific inhibitors. Both strains, albeit to different extents, initiate a maturation program in moDCs as revealed by up-regulation of the cell-surface receptors CD86 and CD197 suggesting additional activation of adaptive immune responses. Furthermore, M. stadtmanae and M. smithii were capable to alter the gene expression of antimicrobial peptides in moDCs to different extents. Taken together, our findings strongly argue that the archaeal gut inhabitants M. stadtmanae and M. smithii are specifically recognized by the human innate immune system. Moreover, both strains are capable of inducing an inflammatory cytokine response to different extents arguing that they might have diverse immunomodulatory functions. In conclusion, we propose that the impact of intestinal methanoarchaea on pathological conditions involving the gut microbiota has been underestimated until now.
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影响因子：
3.7
作者：
Blais Lecours P;Duchaine C;Taillefer M;Tremblay C;Veillette M;Cormier Y;Marsolais D
通讯作者：
Marsolais D
DOI：
10.1099/ijs.0.033712-0
发表时间：
2012-08-01
影响因子：
2.8
作者：
Dridi, Bedis;Fardeau, Marie-Laure;Drancourt, Michel
通讯作者：
Drancourt, Michel
影响因子：
4.9
作者：
Bang, C.;Schilhabel, A.;Schmitz, R. A.
通讯作者：
Schmitz, R. A.
DOI：
10.1177/0968051907079122
发表时间：
2007-01-01
期刊：
JOURNAL OF ENDOTOXIN RESEARCH
影响因子：
--
作者：
Heine, Holger;Gronow, Sabine;Brade, Helmut
通讯作者：
Brade, Helmut
影响因子：
5
作者：
HART D N J;MCKENZIE J L
通讯作者：
MCKENZIE J L