Proteomic and metabolomic analysis of the carotenogenic yeast Xanthophyllomyces dendrorhous using different carbon sources.
Proteomic and metabolomic analysis of the carotenogenic yeast Xanthophyllomyces dendrorhous using different carbon sources.
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DOI：
10.1186/s12864-015-1484-6
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发表时间：
2015-04-12
期刊：
影响因子：
4.4
通讯作者：
Cifuentes V
中科院分区：
文献类型：
--
作者：
Martinez-Moya P;Niehaus K;Alcaíno J;Baeza M;Cifuentes V
虾青素是一种具有生物活性的抗氧化剂。在这种色素的天然来源Xanthophyllomyces dendrohorhous中，胡萝卜素的生成是一个复杂的过程，通过几种机制进行调节，包括碳源。X.当在不可发酵的碳源上生长时，树状酵母产生更多的虾青素，而在高葡萄糖浓度存在下观察到虾青素产量降低。在本研究中，我们使用了比较蛋白质组学和代谢组学分析来表征酵母反应时，在基本培养基中补充葡萄糖（可发酵）或琥珀酸（非发酵）。从蛋白质组学图谱中共鉴定出329个蛋白质，其中大部分蛋白质与胡萝卜素生成、脂质和碳水化合物代谢、氧化还原和应激反应有关。代谢物谱显示92种代谢物主要与糖酵解、三羧酸循环、氨基酸、有机酸、糖和磷酸盐相关。我们确定了丰富的蛋白质和代谢产物的酵母代谢的中心途径，并检查这些分子对胡萝卜素的影响。类似于以前的蛋白质组学应激反应研究，我们观察到调制的丰度从几个氧化还原，应激反应，碳水化合物和脂质酶。此外，海藻糖的积累，关键的ROS反应酶的缺乏，戊糖磷酸途径和三羧酸循环的代谢产物的丰度增加，表明在酵母中的虾青素的积累和氧化应激之间的关联。此外，我们观察到在琥珀酸盐生长条件下的虾青素积累过程中，晚期胡萝卜素生成酶的丰度增加。琥珀酸作为碳源在X.与葡萄糖相比，树状培养物增加了用于虾青素生产的乙酰辅酶A的可用性，这可能反映了三羧酸和乙醛酸循环的代谢酶的正调节。该途径中产生的高代谢物水平可以增加细胞呼吸速率，产生活性氧，从而诱导胡萝卜素的生成。本文的在线版本（doi：10.1186/s12864-015-1484-6）包含补充材料，可供授权用户使用。
Astaxanthin is a potent antioxidant with increasing biotechnological interest. In Xanthophyllomyces dendrorhous, a natural source of this pigment, carotenogenesis is a complex process regulated through several mechanisms, including the carbon source. X. dendrorhous produces more astaxanthin when grown on a non-fermentable carbon source, while decreased astaxanthin production is observed in the presence of high glucose concentrations. In the present study, we used a comparative proteomic and metabolomic analysis to characterize the yeast response when cultured in minimal medium supplemented with glucose (fermentable) or succinate (non-fermentable). A total of 329 proteins were identified from the proteomic profiles, and most of these proteins were associated with carotenogenesis, lipid and carbohydrate metabolism, and redox and stress responses. The metabolite profiles revealed 92 metabolites primarily associated with glycolysis, the tricarboxylic acid cycle, amino acids, organic acids, sugars and phosphates. We determined the abundance of proteins and metabolites of the central pathways of yeast metabolism and examined the influence of these molecules on carotenogenesis. Similar to previous proteomic-stress response studies, we observed modulation of abundance from several redox, stress response, carbohydrate and lipid enzymes. Additionally, the accumulation of trehalose, absence of key ROS response enzymes, an increased abundance of the metabolites of the pentose phosphate pathway and tricarboxylic acid cycle suggested an association between the accumulation of astaxanthin and oxidative stress in the yeast. Moreover, we observed the increased abundance of late carotenogenesis enzymes during astaxanthin accumulation under succinate growth conditions. The use of succinate as a carbon source in X. dendrorhous cultures increases the availability of acetyl-CoA for the astaxanthin production compared with glucose, likely reflecting the positive regulation of metabolic enzymes of the tricarboxylic acid and glyoxylate cycles. The high metabolite level generated in this pathway could increase the cellular respiration rate, producing reactive oxygen species, which induces carotenogenesis. The online version of this article (doi:10.1186/s12864-015-1484-6) contains supplementary material, which is available to authorized users.
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影响因子：
2
作者：
Giardina BJ;Stanley BA;Chiang HL
通讯作者：
Chiang HL
影响因子：
3.7
作者：
Alcaíno J;Romero I;Niklitschek M;Sepúlveda D;Rojas MC;Baeza M;Cifuentes V
通讯作者：
Cifuentes V
影响因子：
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通讯作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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