Comparison of performances of different fungal laccases in delignification and detoxification of alkali-pretreated corncob for bioethanol production.
Comparison of performances of different fungal laccases in delignification and detoxification of alkali-pretreated corncob for bioethanol production.
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不同真菌漆酶对碱预处理玉米芯生物乙醇生产的脱木素和脱毒性能比较
DOI：
10.1093/jimb/kuab013
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发表时间：
2021-04-30
影响因子：
3.4
通讯作者：
中科院分区：
文献类型：
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作者：
摘要 评价碱性真菌漆酶 PIE5 (pH 8.5) 在碱预处理玉米芯脱木素和解毒生产生物乙醇中的性能，并与中性对应物 (rLcc9, 6.5) 进行比较，并以酸性漆酶 rLacA (4.0) 作为独立对照。与各自的对照相比，用三种漆酶处理促进了生物乙醇的产生。经过PIE5、rLcc9和rLacA处理后，碱处理玉米芯的木质素含量分别从4.06%、5.06%和7.80%降低至3.44%、3.95%和5.03%。然而，在降低总苯酚浓度（0.18、0.36 和 0.67 g/l）、提高乙醇浓度（8.02、7.51 和 7.31 g/l）以及体积乙醇生产率（1.34、0.94 和 0.91 g/l hr）方面，漆酶的性能按 rLacA > rLcc9 > PIE5 的顺序排列。缩短整体发酵时间。我们的结果将为未来改进乙醇生产漆酶的尝试提供信息。此外，根据我们的数据以及中性/碱性真菌漆酶处理过程中需要额外程序（例如pH调节）的事实，我们建议在生物乙醇生产中酸性真菌漆酶可能比中性/碱性真菌漆酶更好的选择。
Abstract The performance of the alkaline fungal laccase PIE5 (pH 8.5) in the delignification and detoxification of alkali-pretreated corncob to produce bioethanol was evaluated and compared with that of the neutral counterpart (rLcc9, 6.5), with the acidic laccase rLacA (4.0) was used as an independent control. Treatment with the three laccases facilitated bioethanol production compared with their respective controls. The lignin contents of alkali-pretreated corncob reduced from 4.06%, 5.06%, and 7.80% to 3.44%, 3.95%, and 5.03%, after PIE5, rLcc9, and rLacA treatment, respectively. However, the performances of the laccases were in the order rLacA > rLcc9 > PIE5 in terms of decreasing total phenol concentration (0.18, 0.36, and 0.67 g/l), boosting ethanol concentration (8.02, 7.51, and 7.31 g/l), and volumetric ethanol productivity (1.34, 0.94, and 0.91 g/l hr), and shortening overall fermentation time. Our results would inform future attempts to improve laccases for ethanol production. Furthermore, based on our data and the fact that additional procedures, such as pH adjustment, are needed during neutral/alkaline fungal laccase treatment, we suggest acidic fungal laccases may be a better choice than neutral/alkaline fungal laccases in bioethanol production.
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影响因子：
3.9
作者：
Gaur, Nisha;Narasimhulu, Korrapati;Pydisetty, Y.
通讯作者：
Pydisetty, Y.
影响因子：
2.1
作者：
Hong, YZ;Xiao, YH;Yu, ZL
通讯作者：
Yu, ZL
影响因子：
11.4
作者：
Jurado, Miguel;Prieto, Alicia;Jesus Martinez, Maria
通讯作者：
Jesus Martinez, Maria
DOI：
10.3891/acta.chem.scand.29b-0691
发表时间：
1975-01-01
期刊：
ACTA CHEMICA SCANDINAVICA SERIES B-ORGANIC CHEMISTRY AND BIOCHEMISTRY
影响因子：
--
作者：
FROEHNER, SC;ERIKSSON, KE
通讯作者：
ERIKSSON, KE
影响因子：
3.4
作者：
Moreno, Antonio D.;Ibarra, David;Ballesteros, Mercedes
通讯作者：
Ballesteros, Mercedes