Curcumin Administered in Combination with Glu-GNPs Induces Radiosensitivity in Transplanted Tumor MDA-MB-231-luc Cells in Nude Mice.
Curcumin Administered in Combination with Glu-GNPs Induces Radiosensitivity in Transplanted Tumor MDA-MB-231-luc Cells in Nude Mice.
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姜黄素与 Glu-GNP 联合给药可诱导裸鼠移植肿瘤 MDA-MB-231-luc 细胞的放射敏感性
DOI：
10.1155/2021/9262453
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发表时间：
2021
影响因子：
--
通讯作者：
Hu C
中科院分区：
文献类型：
--
作者：
Li M;Lin L;Guo T;Wu Y;Lin J;Liu Y;Yang K;Hu C
姜黄素是从姜黄根茎中提取的一种植物多酚，具有体外抗乳腺癌细胞活性。为了探讨姜黄素和葡萄糖-金纳米粒(Glu-GNPs)的联合作用及其可能机制，我们在裸鼠体内进行了乳腺癌移植瘤的放射增敏实验。将萤火虫荧光素酶标记的MDA-MB-231细胞接种于裸鼠乳房脂肪垫内，建立人乳腺癌移植瘤模型。荷瘤小鼠在放疗前用不同的药物(姜黄素、Glu-GNPs和顺铂)治疗3周。定期测量小鼠体重和肿瘤体积。使用体内生物发光成像系统实时测定肿瘤生物发光强度，以监测肿瘤生长。移植瘤组织行苏木精-伊红染色，采用逆转录-定量聚合酶链式反应或免疫组织化学方法检测肿瘤组织中血管内皮生长因子、热休克蛋白90、缺氧诱导因子-1α和基质金属蛋白酶9的表达。结果表明，荧光素酶稳定表达，成功建立了荷瘤裸鼠模型。姜黄素可抑制小鼠肿瘤生长，但不会导致小鼠体重显著减轻。此外，姜黄素与Glu-GNPs和照射联合应用时，表现出相加的抑制作用。姜黄素、Glu-GNPs和放射治疗后，模型组肿瘤生物发光强度降低。HE染色显示移植瘤为恶性，中心可见坏死组织。与模型组比较，姜黄素联合谷氨酸-GNPs和X射线照射可降低肿瘤组织中血管内皮生长因子、热休克蛋白90、缺氧诱导因子-1α和基质金属蛋白酶9的蛋白表达。姜黄素和Glu-GNPs联合X射线照射可显著抑制肿瘤生长并诱导放射增敏，其机制可能与抑制肿瘤组织血管生成有关。
Curcumin is a type of plant polyphenol extracted from Curcuma longa L. rhizome, which demonstrates antitumor activity in breast cancer cells in vitro. To investigate the combined effect and possible mechanism of curcumin and glucose-gold nanoparticles (Glu-GNPs), the radiosensitivity of breast carcinoma xenografts was assessed in nude mice. MDA-MB-231 cells labeled with firefly luciferase were inoculated into the mammary fatty pads of nude mice to establish a transplantation tumor model of human breast cancer. The tumor-bearing mice were treated with different drugs (curcumin, Glu-GNPs, and cisplatin) for 3 weeks prior to radiotherapy. The body weights and tumor volumes of the mice were measured in regular intervals. Tumor bioluminescence intensity was determined in real-time using an in vivo bioluminescence imaging system to monitor tumor growth. Transplanted tumor tissue samples were taken for hematoxylin and eosin (HE) staining, and the expression of VEGF, HSP90, HIF-1α, and MMP9 was evaluated via reverse transcription-quantitative PCR or immunohistochemistry. The results revealed that the breast tumor-bearing nude mouse model was successfully established, as evidenced by a stable expression of luciferase. Curcumin inhibited the growth of tumors without causing significant weight loss in mice. Furthermore, additive inhibition was demonstrated when curcumin was administered in combination with Glu-GNPs and irradiation. Tumor bioluminescence intensity was decreased in the model group following curcumin, Glu-GNPs, and irradiation treatment. HE staining demonstrated that transplanted tumors were malignant, with necrotic tissue exhibited centrally. It was concluded that curcumin administered in combination with Glu-GNPs and X-ray irradiation could reduce the protein expression of VEGF, HSP90, HIF-1α, and MMP9 in tumor tissue when compared with the model group. Curcumin and Glu-GNPs administered with X-ray irradiation significantly inhibited tumor growth and induced radiosensitivity, which may be associated with the inhibition of angiogenesis in tumor tissue.
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影响因子：
8
作者：
Hu C;Niestroj M;Yuan D;Chang S;Chen J
通讯作者：
Chen J
影响因子：
13.3
作者：
Kong, Tao;Zeng, Jie;Xing, James Z.
通讯作者：
Xing, James Z.
DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL
影响因子：
2.8
作者：
Grisez, Brian T.;Ray, Justin J.;Lindsey, Brock A.
通讯作者：
Lindsey, Brock A.
影响因子：
3.3
作者：
Shen, Yuxiu;Han, Zaigang;Yuan, Hongyan
通讯作者：
Yuan, Hongyan