The special neuraminidase stalk-motif responsible for increased virulence and pathogenesis of H5N1 influenza A virus.
The special neuraminidase stalk-motif responsible for increased virulence and pathogenesis of H5N1 influenza A virus.
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导致 H5N1 甲型流感病毒毒力和发病机制增加的特殊神经氨酸酶茎基序
DOI：
10.1371/journal.pone.0006277
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发表时间：
2009-07-17
期刊：
影响因子：
3.7
通讯作者：
Jin M
中科院分区：
文献类型：
--
作者：
Zhou H;Yu Z;Hu Y;Tu J;Zou W;Peng Y;Zhu J;Li Y;Zhang A;Yu Z;Ye Z;Chen H;Jin M
高致病性H5 N1禽流感病毒变异导致禽类毒力逐渐增强，人间病例不断积累。流感病毒的神经氨酸酶（NA）茎区变异很大，可能与其毒力有关。所有N1亚型甲型流感病毒的NA茎区可分为六种不同的茎基序，H5 N1/2004样（NA-wt）、WSN样、H5 N1/97样、PR/8样、H7 N1/99样和H5 N1/96样。NA-wt是2000年首次在H5 N1流感病毒中发现的一种特殊的NA茎基序，在茎区的第49 - 68位缺失了20个氨基酸。在这里，我们发现，有一个逐渐增加的特殊NA茎基序在H5 N1分离株从2000年至2007年，特别是，观察到特殊茎基序在所有173人从2004年至2007年的H5 N1分离株。具有特殊茎基序的重组H5 N1病毒在鸡和小鼠中具有最高的毒力和致病性，而具有其他茎基序的重组病毒则表现出减毒表型。这表明自2000年以来，特殊的茎基序有助于H5 N1分离株的高毒力和致病性。在H5 N1分离株中，特别是在人类分离株中，逐渐增加的特殊NA茎基序的出现值得所有人关注。
The variation of highly pathogenic avian influenza H5N1 virus results in gradually increased virulence in poultry, and human cases continue to accumulate. The neuraminidase (NA) stalk region of influenza virus varies considerably and may associate with its virulence. The NA stalk region of all N1 subtype influenza A viruses can be divided into six different stalk-motifs, H5N1/2004-like (NA-wt), WSN-like, H5N1/97-like, PR/8-like, H7N1/99-like and H5N1/96-like. The NA-wt is a special NA stalk-motif which was first observed in H5N1 influenza virus in 2000, with a 20-amino acid deletion in the 49th to 68th positions of the stalk region. Here we show that there is a gradual increase of the special NA stalk-motif in H5N1 isolates from 2000 to 2007, and notably, the special stalk-motif is observed in all 173 H5N1 human isolates from 2004 to 2007. The recombinant H5N1 virus with the special stalk-motif possesses the highest virulence and pathogenicity in chicken and mice, while the recombinant viruses with the other stalk-motifs display attenuated phenotype. This indicates that the special stalk-motif has contributed to the high virulence and pathogenicity of H5N1 isolates since 2000. The gradually increasing emergence of the special NA stalk-motif in H5N1 isolates, especially in human isolates, deserves attention by all.
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