Physalis floridana Cell Number Regulator1 encodes a cell membrane-anchored modulator of cell cycle and negatively controls fruit size.
Physalis floridana Cell Number Regulator1 encodes a cell membrane-anchored modulator of cell cycle and negatively controls fruit size.
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Physalis floriidana Cell Number Regulator1 编码细胞周期的细胞膜锚定调节剂并负控制果实大小。
DOI：
10.1093/jxb/eru415
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发表时间：
2015-01
影响因子：
6.9
通讯作者：
He C
中科院分区：
文献类型：
--
作者：
Li Z;He C
亮点：一种细胞膜锚定蛋白控制子房细胞分裂，并通过MADS结构域蛋白介导决定浆果大小，这表明植物子房身份与生长之间存在分子联系。 酸浆属植物在浆果大小上表现出显著的差异;然而，其潜在的分子基础尚不清楚。在这项工作中，我们发现，在卵巢细胞分裂的差异可能有助于最终浆果大小的变化在酸浆属物种，和mRNA丰度的酸浆细胞数量调节器1（PfCNR 1），番茄果实重量2.2（FW 2.2）的推定直系同源物，与卵巢细胞分裂呈负相关。此外，异时性表达变化的PfCNR 1基因在卵巢伴随浆果重量的变化在酸浆属物种。在转基因酸浆中，多个器官的大小可以通过改变PfCNR 1水平来负控制，并且细胞分裂而不是细胞扩增主要受到影响。PfCNR 1锚定在质膜上，并与PfAG 2（一种决定卵巢身份的AGAMOUS样蛋白）相互作用。PfCYCD 2的表达; 1与PfCNR 1 mRNA水平呈负相关。发现PfAG 2选择性地结合PfCYCD 2;1启动子中的CArG盒并抑制PfCYCD 2;1表达，从而表明PfCNR 1通过PfAG 2介导的途径来调节细胞分裂。PfCNR 1与PfAG 2的相互作用增强了PfCYCD 2;1表达的抑制。PfAG 2的核输入在所提出的途径中是必不可少的。我们的数据为细胞膜锚定蛋白的发育途径提供了新的见解，该蛋白调节细胞分裂并控制器官大小的确定。这项研究也揭示了植物器官身份和器官生长之间的联系。
Highlight: A cell membrane-anchored protein governs ovary cell division and determines berry size mediated by a MADS-domain protein, suggesting a molecular link between ovary identity and growth in plants. Physalis species show a significant variation in berry size; however, the underlying molecular basis is unknown. In this work, we showed that cell division difference in the ovaries might contribute to the ultimate berry size variation within Physalis species, and that mRNA abundance of Physalis floridana Cell Number Regulator1 (PfCNR1), the putative orthologue of the tomato fruit weight 2.2 (FW2.2), was negatively correlated with cell division in the ovaries. Moreover, heterochronic expression variation of the PfCNR1 genes in the ovaries concomitantly correlated with berry weight variation within Physalis species. In transgenic Physalis, multiple organ sizes could be negatively controlled by altering PfCNR1 levels, and cell division instead of cell expansion was primarily affected. PfCNR1 was shown to be anchored in the plasma membrane and to interact with PfAG2 (an AGAMOUS-like protein determining ovary identity). The expression of PfCYCD2;1, a putative orthologue of the mitosis-specific gene CyclinD2;1 in the cell cycle was negatively correlated with the PfCNR1 mRNA levels. PfAG2 was found to selectively bind to the CArG-box in the PfCYCD2;1 promoter and to repress PfCYCD2;1 expression, thus suggesting a PfAG2-mediated pathway for PfCNR1 to regulate cell division. The interaction of PfCNR1 with PfAG2 enhanced the repression of PfCYCD2;1 expression. The nuclear import of PfAG2 was essential in the proposed pathway. Our data provide new insights into the developmental pathways of a cell membrane-anchored protein that modulates cell division and governs organ size determination. This study also sheds light on the link between organ identity and organ growth in plants.
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影响因子：
11.6
作者：
Chen, Yi-Fang;Li, Li-Qin;Wu, Wei-Hua
通讯作者：
Wu, Wei-Hua
影响因子：
30.8
作者：
Cong, Bin;Barrero, Luz S.;Tanksley, Steven D.
通讯作者：
Tanksley, Steven D.
DOI：
10.1073/pnas.0607703104
发表时间：
2007-02-27
影响因子：
11.1
作者：
Nakagawa, Yuko;Katagiri, Takeshi;Iida, Hidetoshi
通讯作者：
Iida, Hidetoshi
DOI：
10.1073/pnas.172520999
发表时间：
2002-10-15
影响因子：
11.1
作者：
Cong, B;Liu, JP;Tanksley, SD
通讯作者：
Tanksley, SD
影响因子：
7.4
作者：
Munos, Stephane;Ranc, Nicolas;Causse, Mathilde
通讯作者：
Causse, Mathilde