Exo-metabolome of Pseudovibrio sp. FO-BEG1 analyzed by ultra-high resolution mass spectrometry and the effect of phosphate limitation.
Exo-metabolome of Pseudovibrio sp. FO-BEG1 analyzed by ultra-high resolution mass spectrometry and the effect of phosphate limitation.
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DOI：
10.1371/journal.pone.0096038
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Schulz-Vogt HN
中科院分区：
文献类型：
--
作者：
Romano S;Dittmar T;Bondarev V;Weber RJ;Viant MR;Schulz-Vogt HN
海洋溶解有机质（DOM）是由生物和非生物作用形成的还原碳化合物的集合体。生物过程包括海洋生物释放或吸收特定分子。异养细菌通常被认为是通过优先吸收某些化合物来影响DOM的组成。然而，它们也会根据生理状态、环境和生长条件分泌多种分子，但迄今为止，这些细菌分泌的全套化合物从未被研究过。在这项研究中，我们分析了外代谢组，代谢产物分泌到环境中，异养海洋细菌Pseudovibrio sp. FO-BEG 1通过超高分辨率质谱，比较磷酸盐限制和磷酸盐过剩的生长条件。属于假弧菌属的细菌已在世界范围内分离出来，主要来自海洋无脊椎动物，并被描述为代谢多功能的α变形菌门。我们表明，外代谢组是出乎意料的大和多样的，由数百种不同的分子式的化合物组成。其特征在于分子的动态再循环，并且其受到菌株的生理状态的显著影响。此外，我们表明，磷酸盐限制极大地影响分泌的分子的量和组成。通过将检测到的质量分配到一般化学类别，我们观察到在磷酸盐过剩条件下分泌的分子主要是肽和高度不饱和化合物。相比之下，在磷酸盐限制下，细菌生长过程中外代谢组的组成发生了变化，表现出高度不饱和，酚类和多酚化合物的增加。最后，我们使用多个代谢物数据库注释了检测到的质量。这些分析表明存在几种与已知生物活性化合物质量类似的质量。然而，仅对一小部分检测到的分子进行了成功的注释，这凸显了目前在海洋异养细菌生物合成能力方面的知识空白。
Oceanic dissolved organic matter (DOM) is an assemblage of reduced carbon compounds, which results from biotic and abiotic processes. The biotic processes consist in either release or uptake of specific molecules by marine organisms. Heterotrophic bacteria have been mostly considered to influence the DOM composition by preferential uptake of certain compounds. However, they also secrete a variety of molecules depending on physiological state, environmental and growth conditions, but so far the full set of compounds secreted by these bacteria has never been investigated. In this study, we analyzed the exo-metabolome, metabolites secreted into the environment, of the heterotrophic marine bacterium Pseudovibrio sp. FO-BEG1 via ultra-high resolution mass spectrometry, comparing phosphate limited with phosphate surplus growth conditions. Bacteria belonging to the Pseudovibrio genus have been isolated worldwide, mainly from marine invertebrates and were described as metabolically versatile Alphaproteobacteria. We show that the exo-metabolome is unexpectedly large and diverse, consisting of hundreds of compounds that differ by their molecular formulae. It is characterized by a dynamic recycling of molecules, and it is drastically affected by the physiological state of the strain. Moreover, we show that phosphate limitation greatly influences both the amount and the composition of the secreted molecules. By assigning the detected masses to general chemical categories, we observed that under phosphate surplus conditions the secreted molecules were mainly peptides and highly unsaturated compounds. In contrast, under phosphate limitation the composition of the exo-metabolome changed during bacterial growth, showing an increase in highly unsaturated, phenolic, and polyphenolic compounds. Finally, we annotated the detected masses using multiple metabolite databases. These analyses suggested the presence of several masses analogue to masses of known bioactive compounds. However, the annotation was successful only for a minor part of the detected molecules, underlining the current gap in knowledge concerning the biosynthetic ability of marine heterotrophic bacteria.
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影响因子：
2.2
作者：
Coucheney, Elsa;Daniell, Tim J.;Nunan, Naoise
通讯作者：
Nunan, Naoise
DOI：
10.1089/15362310260256882
发表时间：
2002-01-01
期刊：
OMICS A Journal of Integrative Biology
影响因子：
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作者：
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通讯作者：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Kattner, Gerhard
影响因子：
4.4
作者：
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Gram, L