Plasmodium falciparum genotype and gametocyte prevalence in children with uncomplicated malaria in coastal Ghana.
Plasmodium falciparum genotype and gametocyte prevalence in children with uncomplicated malaria in coastal Ghana.
复制标题
DOI：
10.1186/s12936-016-1640-8
复制
发表时间：
2016-12-09
期刊：
影响因子：
3
通讯作者：
Amoah LE
中科院分区：
文献类型：
--
作者：
Ayanful-Torgby R;Oppong A;Abankwa J;Acquah F;Williamson KC;Amoah LE
恶性疟原虫配子体对维持疟疾传播至关重要。寄生虫密度、感染复数以及无性基因型是已发现影响配子体产生的特征。测量疟原虫配子体的流行率可作为监测消灭疟疾工作成功与否的一种工具。从2014年6月至8月在加纳南部的三个卫生设施就诊的112名年龄在6个月至13岁之间的患有无并发症恶性疟原虫疟疾的儿童中收集全血，在抗疟疾药物治疗之前（第0天）和完成后4天（第7天）。通过显微镜和聚合酶链反应（PCR）观察疟原虫;通过Pfs 25（PF 3D7_1031000）mRNA真实的时间逆转录聚合酶链反应（RT-PCR）测量亚显微配子体携带。通过使用PCR扩增msp 1（PF3D7_0930300）和msp 2（PF3D7_0206800）的多态性区域，对从干燥滤纸血印迹中提取的gDNA进行寄生虫基因分型。显微镜检查估计，第0天总群体中有3.1%（3/96）携带配子体，第7天降至2.1%（2/96）。相比之下，使用Pfs 25特异性引物（CT < 35）对35个样本的亚组进行逆转录酶-实时PCR（RT-PCR）分析，估计第0天亚显微配子体携带率高达77%（27/35），到第7天仅下降至60%（21/35）。对msp 2基因进行基因分型，发现MOI水平高于msp 1基因。虽然低于检测显微镜，配子体患病率在亚显微镜水平是高的，在这一地区，并强调需要更有效的消除方法，如传播阻断疫苗和更安全的杀配子体药物的发展。本文的在线版本（doi：10.1186/s12936-016-1640-8）包含补充材料，可供授权用户使用。
Plasmodium falciparum gametocytes are vital to sustaining malaria transmission. Parasite densities, multiplicity of infection as well as asexual genotype are features that have been found to influence gametocyte production. Measurements of the prevalence of Plasmodium sp. gametocytes may serve as a tool to monitor the success of malaria eradication efforts. Whole blood was collected from 112 children aged between 6 months and 13 years with uncomplicated P. falciparum malaria attending three health facilities in southern Ghana from June to August, 2014 before (day 0) and 4 days after completion of anti-malaria drug treatment (day 7). Malaria parasites were observed by microscopy and polymerase chain reaction (PCR); submicroscopic gametocyte carriage was measured by a Pfs25 (PF3D7_1031000) mRNA real time reverse transcriptase polymerase chain reaction (RT-PCR). Parasite genotyping was performed on gDNA extracted from dried filter paper blood blots by amplification of the polymorphic regions of msp1 (PF3D7_0930300) and msp2 (PF3D7_0206800) using PCR. Microscopy estimated 3.1% (3/96) of the total population to carry gametocytes on day 0, which decreased to 2.1% (2/96) on day 7. In contrast, reverse transcriptase-real time PCR (RT-PCR) analysis of a subset of 35 samples estimated submicroscopic gametocyte carriage to be as high as 77% (27/35) using primers specific for Pfs25 (CT < 35) on day 0 and by day 7 this only declined to 60% (21/35). Genotyping the msp2 gene identified higher levels of MOI than the msp1 gene. Although below detection by microscopy, gametocyte prevalence at submicroscopic levels are high in this region and emphasize the need for more effective elimination approaches like the development of transmission-blocking vaccines and safer gametocytocidal drugs. The online version of this article (doi:10.1186/s12936-016-1640-8) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
3
作者：
Chen, Ingrid;Poirot, Eugenie;Rooney, Luke
通讯作者：
Rooney, Luke
影响因子：
3
作者：
Baidjoe A;Stone W;Ploemen I;Shagari S;Grignard L;Osoti V;Makori E;Stevenson J;Kariuki S;Sutherland C;Sauerwein R;Cox J;Drakeley C;Bousema T
通讯作者：
Bousema T
DOI：
10.1073/pnas.0500078102
发表时间：
2005-05-24
影响因子：
11.1
作者：
de Roode, JC;Pansini, R;Read, AF
通讯作者：
Read, AF
影响因子：
--
作者：
Duru, Kimberley C;Thomas, Bolaji N
通讯作者：
Thomas, Bolaji N
影响因子：
3
作者：
Agyeman-Budu A;Brown C;Adjei G;Adams M;Dosoo D;Dery D;Wilson M;Asante KP;Greenwood B;Owusu-Agyei S
通讯作者：
Owusu-Agyei S