Trends in multiplicity of Plasmodium falciparum infections among asymptomatic residents in the middle belt of Ghana.
Trends in multiplicity of Plasmodium falciparum infections among asymptomatic residents in the middle belt of Ghana.
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DOI：
10.1186/1475-2875-12-22
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发表时间：
2013-01-17
期刊：
影响因子：
3
通讯作者：
Owusu-Agyei S
中科院分区：
文献类型：
--
作者：
Agyeman-Budu A;Brown C;Adjei G;Adams M;Dosoo D;Dery D;Wilson M;Asante KP;Greenwood B;Owusu-Agyei S
疟疾是加纳中部Kintampo地区儿童死亡和发病的最重要原因。本研究通过研究多态性恶性疟原虫抗原裂殖子表面蛋白2（MSP 2）的分布，调查了金丹波地区无症状居民的感染复数（MOI），以及年龄和季节性对MOI的影响。从2003年11月至2004年10月期间感染恶性疟原虫的无症状儿童和成人队列中提取DNA。进行聚合酶链反应并测定感染复数（MOI）。10岁以下儿童的平均MOI为2.3，而18岁及以上成人的平均MOI为1.4。五岁以下儿童的平均MOI在三月/四月的调查中为2.8，在五月/六月的调查中为0.9。在整个队列中观察到MOI月分布的类似趋势。IC/3D 7菌株在全年的数量超过FC 27菌株，比例约为4：1，在雨季开始的3月/4月调查中，两种菌株的流行率之间的差异最小。MOI与以昆虫学接种率衡量的疟疾传播水平无关。对无症状居民的寄生虫多样性进行基线研究以来采取的干预措施的影响将是进一步调查的主题。
Malaria is the most important cause of mortality and morbidity in children living in the Kintampo districts in the middle part of Ghana. This study has investigated the multiplicity of infection (MOI) within asymptomatic residents of the Kintampo districts, and the influence of age and seasonality on MOI, by studying the distribution of the polymorphic Plasmodium falciparum antigen merozoite surface protein 2 (MSP2). DNA was extracted from an asymptomatic cohort of children and adults infected with P. falciparum during the period November 2003 to October 2004. Polymerase chain reaction was carried out and multiplicity of infection (MOI) was determined. Children under 10 years of age had an average MOI of 2.3 while adults 18 years and above had an average MOI of 1.4. Children below five years had high and low average MOIs of 2.8 in the March/April survey and 0.9 in the May/June survey respectively. A similar trend in the monthly distribution of MOI was observed for the entire cohort. IC/3D7 strains outnumbered the FC27 strains throughout the year by a ratio of about 4:1 with the difference between the prevalence of the two strains being least marked in the March/April survey, at the beginning of the rainy season. MOI was not linked to the level of malaria transmission as measured by the entomological inoculation rate. The impact of interventions, introduced since this baseline study was carried out on the parasite diversity of asymptomatic residents will be the subject of further investigations.
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DOI：
10.1016/s0035-9203(99)90324-3
发表时间：
1999-02-01
影响因子：
2.2
作者：
Felger, I;Smith, T;Beck, HP
通讯作者：
Beck, HP
DOI：
10.4269/ajtmh.1995.52.81
发表时间：
1995-01-01
影响因子：
3.3
作者：
NTOUMI, F;CONTAMIN, H;MERCEREAUPUIJALON, O
通讯作者：
MERCEREAUPUIJALON, O
DOI：
10.1016/0035-9203(94)90391-3
发表时间：
1994-07-01
影响因子：
2.2
作者：
BINKA, FN;MORRIS, SS;ARYEETEY, ME
通讯作者：
ARYEETEY, ME
影响因子：
2.7
作者：
Engelbrecht, F;Tögel, E;Felger, I
通讯作者：
Felger, I
DOI：
10.4269/ajtmh.1996.54.325
发表时间：
1996-04-01
影响因子：
3.3
作者：
Roper, C;Elhassan, IM;Arnot, DE
通讯作者：
Arnot, DE