Contact-free scanning and imaging with the scanning ion conductance microscope.
Contact-free scanning and imaging with the scanning ion conductance microscope.
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DOI:
10.1021/ac402748j
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发表时间:
2014-03-04
影响因子:
7.4
通讯作者:
Moss, Guy
中科院分区:
文献类型:
--
作者:
Del Linz, Samantha;Willman, Eero;Caldwell, Matthew;Klenerman, David;Fernandez, Anibal;Moss, Guy
Scanning ion conductance microscopy (SICM) offers the ability to obtain very high-resolution topographical images of living cells. One of the great advantages of SICM lies in its ability to perform contact-free scanning. However, it is not yet clear when the requirements for this scan mode are met. We have used finite element modeling (FEM) to examine the conditions for contact-free scanning. Our findings provide a framework for understanding the contact-free mode of SICM and also extend previous findings with regard to SICM resolution. Finally, we demonstrate the importance of our findings for accurate biological imaging.
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