Contact-free scanning and imaging with the scanning ion conductance microscope.

Contact-free scanning and imaging with the scanning ion conductance microscope.
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DOI:
10.1021/ac402748j
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发表时间:
2014-03-04
影响因子:
7.4
通讯作者:
Moss, Guy
Moss, Guy
中科院分区:
化学1区
文献类型:
--
作者:
Del Linz, Samantha;Willman, Eero;Caldwell, Matthew;Klenerman, David;Fernandez, Anibal;Moss, Guy

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扫描离子电导显微镜(SICM)提供了获得非常高分辨率的活细胞形貌图像的能力。SICM的最大优势之一在于它能够执行非接触式扫描。但是,目前尚不清楚何时满足这种扫描模式的要求。我们已经使用有限元建模(FEM)来检查无接触扫描的条件。我们的研究结果提供了一个框架,了解无接触模式的SICM,也扩展了以前的研究结果与SICM决议。最后,我们证明了我们的研究结果的重要性,准确的生物成像。
Scanning ion conductance microscopy (SICM) offers the ability to obtain very high-resolution topographical images of living cells. One of the great advantages of SICM lies in its ability to perform contact-free scanning. However, it is not yet clear when the requirements for this scan mode are met. We have used finite element modeling (FEM) to examine the conditions for contact-free scanning. Our findings provide a framework for understanding the contact-free mode of SICM and also extend previous findings with regard to SICM resolution. Finally, we demonstrate the importance of our findings for accurate biological imaging.
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