Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics.
Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics.
复制标题
DOI：
10.1016/j.isci.2023.108024
复制
发表时间：
2023-11-17
期刊：
影响因子：
5.8
通讯作者：
Zhuo Y
中科院分区：
文献类型：
--
作者：
Jia X;Wu J;Chen X;Hou S;Li Y;Zhao L;Zhu Y;Li Z;Deng C;Su W;Zhuo Y
小梁网是房水流出的主要通道，其功能障碍可导致眼压升高，从而诱发原发性开角型青光眼（POAG）。本研究采用单细胞RNA测序（scRNA-seq）技术构建图谱，进一步探讨自发性POAG和健康猕猴与TM收缩功能障碍相关的细胞异质性。我们建立了TM图谱，鉴定出14种不同的细胞类型。在Beam A， Beam B， Beam C和平滑肌细胞（SMC）细胞类型中，我们首次发现了与TM收缩相关的多个基因（例如TPM1， ACTC1, TNNT1），并确定了它们在POAG组和健康组中的差异表达。此外，观察到POAG非人灵长类动物的TM微结构变化，其致密和塌陷。因此，我们的研究表明TPM1可能是调节TM结构、收缩功能和房水流出阻力的关键靶点。建立青光眼猕猴和健康猕猴小梁网细胞图谱揭示POAG猕猴小梁网微结构变化的详细特征探讨小梁网功能障碍的差异表达基因及通路说明收缩期功能障碍可能在POAG视光发展中起重要作用；转录组
As the major channel of aqueous humor outflow, dysfunction of trabecular meshwork (TM) can lead to intraocular pressure elevating, which can trigger primary open-angle glaucoma (POAG). In this study, we use single-cell RNA sequencing (scRNA-seq) technique to build an atlas and further explore the spontaneous POAG and healthy macaques cellular heterogeneity associated with the dysfunction of TM contraction. We built the TM atlas, which identified 14 different cell types. In Beam A, Beam B, Beam C, and smooth muscle cell (SMC) cell types, we first found multiple genes associated with TM contraction (e.g., TPM1, ACTC1, TNNT1), determining their differential expression in the POAG and healthy groups. In addition, the microstructural alterations in TM of POAG non-human primates were observed, which was compact and collapsed. Thus, our study indicated that TPM1 may be a key target for regulating TM structure, contraction function, and resistance of aqueous humor outflow. Establish a cell atlas for trabecular meshwork (TM) of glaucoma and healthy macaques Showed the detailed features of microstructural alterations in TM of POAG macaques Explore the differentially expressed genes and pathways with the dysfunction of TM Illustrate that systolic dysfunction may play an essential role in developing POAG Optometry; Transcriptomics
登录
查看更多内容
Bmi-1高表达细胞丰富心脏干/祖细胞并对心脏损伤做出反应
影响因子：
5.3
作者：
Song Y;Zhao M;Xie Y;Zhu T;Liang W;Sun B;Liu W;Wu L;Lu G;Li TS;Yin T;Xie Y
通讯作者：
Xie Y
与年龄相关的后睫状肌肉限制 - 小梁网与视神经头部病理生理学之间的联系。
影响因子：
3.4
作者：
Croft MA;Lütjen-Drecoll E;Kaufman PL
通讯作者：
Kaufman PL
DOI：
10.1126/science.1247651
发表时间：
2014-02-14
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Jaitin DA;Kenigsberg E;Keren-Shaul H;Elefant N;Paul F;Zaretsky I;Mildner A;Cohen N;Jung S;Tanay A;Amit I
通讯作者：
Amit I
影响因子：
2.4
作者：
McGrady NR;Minton AZ;Stankowska DL;He S;Jefferies HB;Krishnamoorthy RR
通讯作者：
Krishnamoorthy RR
影响因子：
5.2
作者：
Meng, Ling-Bing;Shan, Meng-Jie;Gong, Tao
通讯作者：
Gong, Tao