siRNA transfection in larvae of the barnacle Amphibalanus amphitrite
siRNA transfection in larvae of the barnacle Amphibalanus amphitrite
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藤壶 Amphibalanus amphitrite 幼虫中的 siRNA 转染
DOI：
10.1242/jeb.120113
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发表时间：
2015-08
影响因子：
2.8
通讯作者：
Pei-Yuan Qian
中科院分区：
文献类型：
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作者：
Gen Zhang;Li-Sheng He;Yue Him Wong;Li Yu;Pei-Yuan Qian
RNA interference (RNAi) provides an efficient and specific technique for functional genomic studies. Yet, no successful application of RNAi has been reported in barnacles. In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either nauplius or cyprid stage, or both. Effects of siRNA transfection on p38 MAPK level were hardly detectable in the cyprids that their corresponding nauplii were transfected. In contrast, larvae that were transfected at cyprid stage showed lower level of p38 MAPK than the blank and reagent controls. However, significantly decreased level of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rate were observed only in the “Double Transfection”, in which larvae were exposed to siRNA solution at both the nauplius and cyprid stages. Relatively longer transfection time and more cells of the larvae exposed to siRNA directly might explain the higher efficiency in the “Double Transfection”.
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影响因子：
3.5
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.0903105106
发表时间：
2009-08-18
影响因子：
11.1
作者：
Liubicich, Danielle M.;Serano, Julia M.;Patel, Nipam H.
通讯作者：
Patel, Nipam H.
影响因子：
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作者：
Takahiro Ohde;M. Masumoto;T. Yaginuma;T. Niimi
通讯作者：
Takahiro Ohde;M. Masumoto;T. Yaginuma;T. Niimi