Increased mandibular condylar growth in mice with estrogen receptor beta deficiency.
Increased mandibular condylar growth in mice with estrogen receptor beta deficiency.
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DOI：
10.1002/jbmr.1835
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发表时间：
2013-05
影响因子：
6.2
通讯作者：
Wadhwa, Sunil
中科院分区：
文献类型：
--
作者：
Kamiya, Yosuke;Chen, Jing;Xu, Manshan;Utreja, Achint;Choi, Thomas;Drissi, Hicham;Wadhwa, Sunil
颞下颌关节（TMJ）疾病主要困扰育龄妇女，这表明女性激素在疾病过程中的作用。在长骨中，雌激素通过雌激素受体β（ER β）发挥作用，抑制雌性小鼠的轴向骨骼生长。然而，ER β在下颌骨髁突中的作用在很大程度上是未知的。我们假设雌激素受体β缺陷小鼠与野生型（WT）雌性小鼠相比，下颌骨髁突生长增加。本研究检查了雌性7、49和120日龄WT和ER β敲除（KO）小鼠。与WT对照组相比，49天和120日龄雌性ER β KO组由于细胞数量增加，下颌髁突软骨厚度显著增加。对49日龄雌性ER β KO小鼠的分析显示，与WT对照组相比，X型胶原、Pthrp和护骨素基因表达显著增加，Rankl和Ihh基因表达显著降低。软骨下骨分析显示，与WT雌性小鼠相比，49日龄ER β KO小鼠的总髁突体积显著增加，破骨细胞数量减少。髁突软骨细胞增殖在基因型间无差异。然而，调节细胞周期的蛋白质表达存在差异;我们发现与WT小鼠相比，ER β KO小鼠下颌髁突软骨中Tieg 1和p57的表达降低。综上所述，我们的研究结果表明，雌激素受体β缺乏通过抑制纤维软骨的周转增加髁突的生长。
Temporomandibular joint (TMJ) disorders predominantly afflict women of childbearing age, suggesting a role for female hormones in the disease process. In long bones, estrogen acting via estrogen receptor beta (ERβ) inhibits axial skeletal growth in female mice. However, the role of ERβ in the mandibular condyle is largely unknown. We hypothesize that female ERβ deficient mice will have increased mandibular condylar growth compared with wild type (WT) female mice. This study examined female 7-, 49- and 120-day-old WT and ERβ knockout (KO) mice. There was a significant increase in mandibular condylar cartilage thickness, due to an increased number of cells, in the 49- and 120-day-old female ERβ KO compared with WT controls. Analysis in 49-day-old female ERβ KO mice revealed a significant increase in collagen type X, Pthrp and osteoprotegrin gene expression and a significant decrease in Rankl and Ihh gene expression, compared with WT controls. Subchondral bone analysis revealed a significant increase in total condylar volume and a decrease in the number of osteoclasts in the 49-day-old ERβ KO compared with WT female mice. There was no difference in cell proliferation in condylar cartilage between the genotypes. However, there were differences in the expression of proteins that regulate the cell cycle; we found a decrease in the expression of Tieg1 and p57 in the mandibular condylar cartilage from ERβ KO mice compared with WT mice. Taken together, our results suggest that ERβ deficiency increases condylar growth in female mice by inhibiting the fibrocartilage turnover.
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影响因子：
6.2
作者：
Chagin, AS;Lindberg, MK;Ohlsson, C
通讯作者：
Ohlsson, C
DOI：
10.1159/000103003
发表时间：
2007-01-01
期刊：
Medicine and sport science
影响因子：
--
作者：
Daly, Robin M
通讯作者：
Daly, Robin M
影响因子：
2.6
作者：
Fujita, T;Ohtani, J;Tanne, K
通讯作者：
Tanne, K
影响因子：
3.8
作者：
Min, H-J;Lee, M-J;Jung, H-S
通讯作者：
Jung, H-S
影响因子：
--
作者：
Hawse, John R.;Subramaniam, Malayannan;Spelsberg, Thomas C.
通讯作者：
Spelsberg, Thomas C.