Comparison between the influence of the systemic and central injection of alcohol on Leydig cell activity.
Comparison between the influence of the systemic and central injection of alcohol on Leydig cell activity.
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全身和中枢注射酒精对 Leydig 细胞活性影响的比较。
DOI：
10.1097/01.alc.0000117839.69352.b3
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Rivier,CatherineL
中科院分区：
文献类型：
--
作者：
Selvage,DanielJ;Hales,DBuchanan;Rivier,CatherineL
Abstract:Background:Systemic alcohol exposure lowers plasma testosterone (T) levels in adult males, but the relative role of impaired luteinizing hormone (LH)‐releasing hormone synthesis and decreased pituitary LH release versus that of a direct ability of circulating alcohol to inhibit testicular steroidogenesis remains poorly understood. We had reported preliminary evidence that alcohol might stimulate a pituitary‐independent, neural pathway between the hypothalamus and the testes whose activation blunts T secretion in response to human chorionic gonadotropin (hCG). The present work was done to further investigate the influence of alcohol on this pathway by comparing the effect of the intragastric (ig) and intracerebroventricular (icv) injection of alcohol on the T response to hCG, to probe the role of LH and corticotropin‐releasing factor (CRF) in both models, and to examine potential changes in levels of the cholesterol transfer protein steroidogenic acute regulatory (StAR) protein.Methods:Male Sprague Dawley® rats were implanted with chronic icv, ig, and/or intravenous cannulae that allowed drug delivery and blood sampling in nonanesthetized, undisturbed animals. T blood levels were measured by radioimmunoassay. The role of LH and of hormones of the hypothalamic‐pituitary‐adrenal axis such as adrenocorticotropic hormone and corticosterone was investigated in rats pretreated with an LH‐releasing hormone antagonist or CRF antibodies. The potential presence of neuronal damage was assessed by Fluoro‐Jade methodology. StAR protein levels were measured by Western blot in Leydig cells isolated from rats injected with the vehicle or alcohol.Results:Although it was not accompanied by measurable blood alcohol levels, icv administered alcohol, at a dose (5 μl of 200 proof, 86 μM) that did not cause neuronal damage and did not lead to detectable levels of the drug in the cerebrospinal fluid of the fourth ventricle of the brain, significantly blunted hCG‐induced T release. The ig injection of alcohol, which in contrast induced significant increases in blood alcohol levels, also significantly interfered with the ability of hCG to induce T release. This effect was comparable in 40‐ and 65‐day‐old rats. Neither prior blockade of LH‐releasing hormone receptors with a potent LH‐releasing hormone antagonist nor immunoneutralization of endogenous CRF altered the inhibitory effect of alcohol injected icv or ig on T secretion. Preliminary results suggested that testicular levels of StAR protein may be slightly decreased by both alcohol regimens.Conclusions:Collectively, our results indicate that alcohol can act within the brain to influence testicular activity independently of LH, independently of hormones of the hypothalamic‐pituitary‐adrenal axis, and/or independently of the presence of the drug in the circulation. Our present working hypothesis is that the icv injection of alcohol stimulates an inhibitory neural pathway that connects the hypothalamus to the testes.
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影响因子：
--
作者：
T. Orr;Taylor Mf;Bhattacharyya Ak;Collins Dc;David R. Mann
通讯作者：
T. Orr;Taylor Mf;Bhattacharyya Ak;Collins Dc;David R. Mann
影响因子：
2.7
作者：
Vetter-O'Hagen, Courtney S.;Spear, Linda P.
通讯作者：
Spear, Linda P.
酒精会迅速降低大鼠的血浆睾酮水平：证据表明神经脑-性腺途径可能对于降低睾丸对促性腺激素的反应性很重要。
DOI：
10.1111/j.1530-0277.1999.tb04021.x
发表时间：
1999
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Rivier,C
通讯作者：
Rivier,C
大鼠脑室内注射 IL-1β 后血液中白细胞介素 6 (IL-6) 水平升高的机制：神经控制与体液控制 1。
影响因子：
4.8
作者：
Guanjie Chen;S. Reichlin
通讯作者：
S. Reichlin
酒精诱导的完整和去性腺切除的雄性和雌性大鼠中 LH 分泌的抑制：可能的机制。
DOI：
10.1111/j.1530-0277.1992.tb01896.x
发表时间：
1992
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Rivier,C;Rivest,S;Vale,W
通讯作者：
Vale,W