Effects of Escherichia coli on mixotrophic growth of Chlorella minutissima and production of biofuel precursors.
Effects of Escherichia coli on mixotrophic growth of Chlorella minutissima and production of biofuel precursors.
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DOI：
10.1371/journal.pone.0096807
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
VanderGheynst JS
中科院分区：
文献类型：
--
作者：
Higgins BT;VanderGheynst JS
在气升式反应器中，在兼养条件下（葡萄糖、甘油和乙酸盐底物），将小球藻与大肠杆菌共培养，以确定细菌污染对藻类生物燃料生产的可能影响。推测E. coli与C. minutissima的养分，取代藻类生物量。然而，C. minutissima在E.大肠杆菌，表明生物体之间的共生关系。在初始底物浓度为1%时，共培养产生的藻类生物量比纯培养多200-587%。微小的文化在1%底物上生长的共培养物消耗的可用碳底物比E. coli和C. minutissima单独。在1%底物下，与纯培养物相比，共培养物中的总脂质和淀粉生产率升高，表明在这种特定情况下细菌污染对生物燃料前体的生产无害。然而，在共培养物中观察到的反应器的生物结垢和在所有兼养培养物中的酸形成可能对放大提出挑战。
Chlorella minutissima was co-cultured with Escherichia coli in airlift reactors under mixotrophic conditions (glucose, glycerol, and acetate substrates) to determine possible effects of bacterial contamination on algal biofuel production. It was hypothesized that E. coli would compete with C. minutissima for nutrients, displacing algal biomass. However, C. minutissima grew more rapidly and to higher densities in the presence of E. coli, suggesting a symbiotic relationship between the organisms. At an initial 1% substrate concentration, the co-culture produced 200-587% more algal biomass than the axenic C. minutissima cultures. Co-cultures grown on 1% substrate consumed 23–737% more of the available carbon substrate than the sum of substrate consumed by E. coli and C. minutissima alone. At 1% substrate, total lipid and starch productivity were elevated in co-cultures compared to axenic cultures indicating that bacterial contamination was not detrimental to the production of biofuel precursors in this specific case. Bio-fouling of the reactors observed in co-cultures and acid formation in all mixotrophic cultures, however, could present challenges for scale-up.
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DOI：
10.1016/j.chroma.2012.04.074
发表时间：
2012-06-29
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Kind T;Meissen JK;Yang D;Nocito F;Vaniya A;Cheng YS;Vandergheynst JS;Fiehn O
通讯作者：
Fiehn O
影响因子：
2.7
作者：
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通讯作者：
Oswald, WJ
影响因子：
64.8
作者：
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通讯作者：
Smith, AG
影响因子：
2.9
作者：
de-Bashan, Luz E.;Antoun, Hani;Bashan, Yoav
通讯作者：
Bashan, Yoav
影响因子：
11.2
作者：
Davis, Ryan;Aden, Andy;Pienkos, Philip T.
通讯作者：
Pienkos, Philip T.