Genetic analysis of loci associated with partial resistance to Sclerotinia sclerotiorum in rapeseed (Brassica napus L.)
Genetic analysis of loci associated with partial resistance to Sclerotinia sclerotiorum in rapeseed (Brassica napus L.)
复制标题
油菜核盘菌部分抗性相关位点的遗传分析（Brassica napus L.）
DOI：
10.1007/s00122-002-1171-2
复制
发表时间：
2003-02
期刊：
影响因子：
--
通讯作者：
赵建伟
中科院分区：
文献类型：
--
作者：
赵建伟
摘要：油菜菌核病是油菜的主要病害之一。在中国利用油菜雄性不育恢复系H5200与部分抗菌核病品系宁RS-1杂交的128个F2：3家系群体，检测了与抗菌核病有关的数量性状位点（QTL）。利用107个分子标记（包括72个RFLP、30个AFLP、3个SSR和2个RAPD标记）构建了一张包含23个连锁群的遗传连锁图谱，图谱覆盖范围为1625.7 cM，平均连锁间距为15.2 cM。在两个发育阶段经验性地评估抗性：在幼苗阶段用离体叶接种，在成熟植物阶段用体内茎接种。将每株植物观察到的抗性评分为苗期的叶抗性（LRS）和成熟植物期的茎抗性（SRM）。单因素方差分析共检测到13个位点，MapMaker-QTL共检测到6个QTL。结果表明，6个QTL中有3个与苗期叶片抗性相关，它们分别占总表型变异的23.2%、16.6%和13.6%，合计贡献率为40.7%。在成熟株期发现了3个与抗病性相关的QTL，解释了49.0%的表型变异。抗性表现上位性，以加性互作为主。说明单位点QTL和上位性互作在油菜抗菌核病中起重要作用。
Abstract.Sclerotinia stem rot is the most devastating disease of rapeseed (Brassica napus L.) in China. Quantitative trait loci (QTLs) involved in resistance to Sclerotinia sclerotiorum were detected in a rapeseed population of 128-F2:3 families derived from a cross between the male sterility restorer line H5200 and a partial resistant line Ning RS-1. A total of 107 molecular markers including 72 RFLPs, 30 AFLPs, 3 SSRs and 2 RAPDs were employed to construct a genetic linkage map with 23 linkage groups covering 1,625.7 cM with an average space of 15.2 cM. Resistance was assessed empirically at two developmental stages: with a detached leaf inoculation at the seedling stage and in vivo stem inoculation at the mature plant stage. The observed resistance was scored for each plant as leaf resistance at the seedling stage (LRS) and stem resistance at the mature plant stage (SRM). A total of 13 loci were identified by one-way ANOVA and six QTLs were detected with MapMaker-QTL. We found that three of the six QTLs were associated with leaf resistance at the seedling stage and collectively accounted for 40.7% of the total phenotypic variation, each accounting for 23.2%, 16.6% and 13.6% respectively. Three QTLs were found corresponding to the disease resistance at the mature plant stage, explaining 49.0% of the phenotypic variation. Epistasis was observed for the resistance and the additive by additive interactions were the predominant type of epistasis. It was concluded that both single-locus QTLs and epistatic interactions played important roles in Sclerotinia resistance in rapeseed.
登录
查看更多内容
影响因子：
3.3
作者：
D. Butruille;R. Guries;Thomas C. Osborn
通讯作者：
D. Butruille;R. Guries;Thomas C. Osborn
影响因子：
11.1
作者：
S. Tanksley
通讯作者：
S. Tanksley
影响因子：
1.3
作者：
Jelena Bradic;Jelena Bradic;Jiaqi Guo;Hooman Sherkat;Johnny Yang;Selene Xu
通讯作者：
Jelena Bradic;Jelena Bradic;Jiaqi Guo;Hooman Sherkat;Johnny Yang;Selene Xu
影响因子：
5.4
作者：
E. Pang;G. Halloran
通讯作者：
E. Pang;G. Halloran
影响因子：
5.4
作者：
M. Uzunova;Wolfgang Ecke;K. Weissleder;G. Robbelen
通讯作者：
M. Uzunova;Wolfgang Ecke;K. Weissleder;G. Robbelen